Overview

  • Product name
    Anti-Cytokeratin 4 antibody
    See all Cytokeratin 4 primary antibodies
  • Description
    Rabbit polyclonal to Cytokeratin 4
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 317-505 of Human Cytokeratin 4.

  • Positive control
    • HeLa whole cell lysate; HeLa cells; Human BT474 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Tissue specificity
    Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.
  • Involvement in disease
    Defects in KRT4 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links
  • Form
    Localisation: Intermediate filament (Cytoskeleton).
  • Alternative names
    • CK 4 antibody
    • CK-4 antibody
    • CK4 antibody
    • CYK4 antibody
    • Cytokeratin 4 antibody
    • Cytokeratin-4 antibody
    • Cytokeratin4 antibody
    • FLJ31692 antibody
    • K2C4_HUMAN antibody
    • K4 antibody
    • Keratin 4 antibody
    • Keratin antibody
    • Keratin type II cytoskeletal 4 antibody
    • Keratin-4 antibody
    • Keratin4 antibody
    • KRT 4 antibody
    • Krt4 antibody
    • type II cytoskeletal 4 antibody
    • Type-II keratin Kb4 antibody
    see all

Images

  • Anti-Cytokeratin 4 antibody (ab154406) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 57 kDa
  • Immunohistochemical analysis of paraffin-embedded Human BT474 xenograft tissue, labeling Cytokeratin 4 with ab154406 at 1/500 dilution.
  • Immunofluorescence analysis of methanol-fixed HeLa cells (iced-cold, 5 min) labeling Cytokeratin 4 with ab154406 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.

References

ab154406 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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