Overview

  • Product nameAnti-Cytokeratin 4 antibody [EPR1598]
    See all Cytokeratin 4 primary antibodies
  • Description
    Rabbit monoclonal [EPR1598] to Cytokeratin 4
  • SpecificityThe immunogen shares high homology with other cytokeratins like cytokeratin-3(93%), cytokeratin-79(85.7%) and cytokeratin-2 oral(85.7%) etc.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues within Human Cytokeratin 4.

  • Positive control
    • WB: A431 and Human uterus cell lysates IHC-P: Human tonsil tissue ICC/IF: A431 cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab92465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
  • Application notesICC/IF: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Antigen retrieval is recommended before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
    WB: 1/1000 - 1/10,000. Predicted molecular weight: 57 kDa.

    Is unsuitable for Flow Cyt or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificityDetected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer.
    • Involvement in diseaseDefects in KRT4 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Information by UniProt
    • Database links
    • FormLocalisation: Intermediate filament (Cytoskeleton).
    • Alternative names
      • CK 4 antibody
      • CK-4 antibody
      • CK4 antibody
      • CYK4 antibody
      • Cytokeratin 4 antibody
      • Cytokeratin-4 antibody
      • Cytokeratin4 antibody
      • FLJ31692 antibody
      • K2C4_HUMAN antibody
      • K4 antibody
      • Keratin 4 antibody
      • Keratin antibody
      • Keratin type II cytoskeletal 4 antibody
      • Keratin-4 antibody
      • Keratin4 antibody
      • KRT 4 antibody
      • Krt4 antibody
      • type II cytoskeletal 4 antibody
      • Type-II keratin Kb4 antibody
      see all

    Anti-Cytokeratin 4 antibody [EPR1598] images

    • All lanes : Anti-Cytokeratin 4 antibody [EPR1598] (ab92465) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : Human uterus cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 57 kDa
    • ab92465, at a 1/100 dilution, staining Cytokeratin 4 in paraffin embedded Human tonsil tissue by Immunohistochemistry. Note positive staining of squamous cells.
    • Immunofluorescent staining of A431 cells, using ab92465 at a 1/100 dilution.

    References for Anti-Cytokeratin 4 antibody [EPR1598] (ab92465)

    This product has been referenced in:
    • Jacot TA  et al. Development of a composite measure of product adherence, protocol compliance, and semen exposure using DNA and protein biomarkers for topical HIV prevention studies. PLoS One 9:e114368 (2014). ICC ; Human . Read more (PubMed: 25489736) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab92465.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"