Overview

  • Product name
    Anti-Cytokeratin 5 + 14 antibody [16.4]
    See all Cytokeratin 5 + 14 primary antibodies
  • Description
    Mouse monoclonal [16.4] to Cytokeratin 5 + 14
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (cytokeratin enriched extract of feline tongue epithelium).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.08% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    16.4
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-Fr
WB
  • Application notes
    ICC/IF: Use at an assay dependent dilution (from PubMed: 17361080)
    IHC-Fr: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of approximately 58 and 50 kDa corresponding to cytokeratins 5 and 14 respectively.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (i.e. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells.
    • Cellular localization
      Cytoplasmic; Basal layer
    • Database links
    • Alternative names
      • CK 14 antibody
      • CK 5 antibody
      • DDD antibody
      • k14 antibody
      • k5 antibody
      • Keratin 14 antibody
      • Keratin 5 antibody
      • Keratin type I cytoskeletal 14 antibody
      • Keratin type II cytoskeletal 5 antibody
      • krt14 antibody
      • krt5 antibody
      see all

    References for Anti-Cytokeratin 5 + 14 antibody [16.4] (ab16570)

    This product has been referenced in:
    • Arthur GK  et al. KCa3.1 K+ Channel Expression and Function in Human Bronchial Epithelial Cells. PLoS One 10:e0145259 (2015). Read more (PubMed: 26689552) »
    • Woodman LB  et al. Synthetic response of stimulated respiratory epithelium: modulation by prednisolone and iKK2 inhibition. Chest 143:1656-66 (2013). ICC/IF ; Human . Read more (PubMed: 23238614) »

    See all 3 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab16570.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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