Anti-Cytokeratin 5 + 14 antibody [LH8] (ab20203)
Key features and details
- Mouse monoclonal [LH8] to Cytokeratin 5 + 14
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Human
- Isotype: IgM
Overview
-
Product name
Anti-Cytokeratin 5 + 14 antibody [LH8]
See all Cytokeratin 5 + 14 primary antibodies -
Description
Mouse monoclonal [LH8] to Cytokeratin 5 + 14 -
Host species
Mouse -
Specificity
Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen. -
Tested applications
Suitable for: IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
The 50,000 Mr band (including K14 )cut from an SDS-polyacrylamide gel and Triton X-100 extracted cytoskeleton pellet from cultured human foreskin keratinocytes.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
-
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
LH8 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgM -
Light chain type
unknown -
Research areas
Associated products
-
Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-Fr |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration. PubMed: 24705787
|
Notes |
---|
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. PubMed: 24705787 |
Target
-
Relevance
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (i.e. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. -
Cellular localization
Cytoplasmic; Basal layer -
Database links
- Entrez Gene: 3852 Human
- Entrez Gene: 3861 Human
- Omim: 148040 Human
- Omim: 148066 Human
- SwissProt: P02533 Human
- SwissProt: P13647 Human
-
Alternative names
- CK 14 antibody
- CK 5 antibody
- DDD antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab20203 has been referenced in 2 publications.
- Moser B et al. Expression of RAGE and HMGB1 in thymic epithelial tumors, thymic hyperplasia and regular thymic morphology. PLoS One 9:e94118 (2014). IHC-P ; Human . PubMed: 24705787
- Prince ME et al. Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma. Proc Natl Acad Sci U S A 104:973-8 (2007). IHC-Fr ; Human . PubMed: 17210912