Anti-Cytokeratin 5 + 14 antibody [XM26 & LL002], prediluted (ab59693)

Overview

  • Product nameAnti-Cytokeratin 5 + 14 antibody [XM26 & LL002], prediluted
    See all Cytokeratin 5 + 14 primary antibodies
  • Description
    Mouse monoclonal [XM26 & LL002] to Cytokeratin 5 + 14, prediluted
  • SpecificityThis antibody cocktail reacts with cytokeratin 5 intermediate filament protein as well as to 50 kD cytokeratin protein designated cytokeratin 14
  • Tested applicationsSuitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    XM26: Prokaryotic recombinant protein corresponding to 103 amino acid portion of C terminal region of the human Cytokeratin 5 molecule. LL002: Synthetic peptide of the extreme C terminal of human Cytokeratin 14.

  • Positive control
    • Skin
  • General notesThe isotypes for both clones are as follows: XM26 - IgG1 LL002 - IgG3


    Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Belonging to the type A (acidic) subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas.

Properties

  • FormPrediluted
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: Inert stabilizer
  • Concentration information loading...
  • Primary antibody notesMonoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Belonging to the type A (acidic) subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas.
  • ClonalityMonoclonal
  • Clone numberXM26 & LL002
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. Incubation for 30 minutes at room temperature is recommended.

Target

  • RelevanceCytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (i.e. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells.
  • Cellular localizationCytoplasmic; Basal layer
  • Database links
  • Alternative names
    • CK 14 antibody
    • CK 5 antibody
    • DDD antibody
    • k14 antibody
    • k5 antibody
    • Keratin 14 antibody
    • Keratin 5 antibody
    • Keratin type I cytoskeletal 14 antibody
    • Keratin type II cytoskeletal 5 antibody
    • krt14 antibody
    • krt5 antibody
    see all

Anti-Cytokeratin 5 + 14 antibody [XM26 & LL002], prediluted images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil tissue sections, labelling Cytokeratin 5/14 with ab59693.

References for Anti-Cytokeratin 5 + 14 antibody [XM26 & LL002], prediluted (ab59693)

ab59693 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"