Anti-Cytokeratin 5 + 6 antibody [D5/16 B4] (ab17133)

Overview

  • Product name
    Anti-Cytokeratin 5 + 6 antibody [D5/16 B4]
  • Description
    Mouse monoclonal [D5/16 B4] to Cytokeratin 5 + 6
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Cytokeratin 5 (Human).

  • Positive control
    • Skin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    D5/16 B4
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance
    Cytokeratin 5 (58 kD) is a high molecular weight, basic type of cytokeratin expressed in basal, the intermediate and the superficial cell layers of stratified epithelia as well as transitional epithelia, complex epithelia and in mesothelial cells and mesothelioma. Cytokeratin 6 (56 kD) is also a high molecular weight, basic type cytokeratin expressed by proliferating squamous epithelium often paired with Cytokeratin 16.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • Cytokeratin 5 antibody
    • Cytokeratin 6A antibody
    • Cytokeratin 6B antibody
    • DDD antibody
    • EBS2 antibody
    • K5 antibody
    • K6A antibody
    • K6B antibody
    • Keratin 5 antibody
    • Keratin 6A antibody
    • Keratin 6B antibody
    • Keratin type II cytoskeletal 5 antibody
    • KRT 5 antibody
    • KRT 5A antibody
    • KRT 6A antibody
    • KRT 6B antibody
    see all

References

This product has been referenced in:
  • Fasano M  et al. Small bowel metastasis from pancreatic cancer in a long-term survival patient with synchronous advanced malignant pleural mesothelioma: A case report and literature review. Oncol Lett 12:4505-4509 (2016). IHC-P ; Human . Read more (PubMed: 28105159) »
  • Wen YH  et al. Id4 protein is highly expressed in triple-negative breast carcinomas: possible implications for BRCA1 downregulation. Breast Cancer Res Treat 135:93-102 (2012). IHC-P ; Human . Read more (PubMed: 22538771) »

See all 4 Publications for this product

Customer reviews and Q&As

Merci de nous avoir contactés.

L'anticorps ab17133 a une concentration de 16µg/ml.

Veuillez noter que le tampon de stockage contient 0.05% d'azoture de sodium et 1% de BSA, 2 éléments qui peuvent poser probl...

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DISCOUNT CODE:100%ABR-EXXXXX
Expiration date: 15/12/2012
I am very pleased to hear you would like to accept our offer and test ab17133 in Mouse. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer...

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Thank you for your enquiry and your interest in our products.
I have the confirmation from the Lab that this antibody has been validated only with human tissue specimens and we do not have any cross reactive with mouse samples.
If you wish to...

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Thank you for confirming that information.

Kate is currently away from the office but I will try to assist you as best I can.

As I am sure you are aware, the antibody purchased, Anti-Cytokeratin 5 + 6 antibody (ab17133) was ordere...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 1.5%
Username

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Verified customer

Submitted Apr 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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