Anti-Cytokeratin 5 + 6 antibody [D5/16 B4] (ab17133)

Overview

  • Product nameAnti-Cytokeratin 5 + 6 antibody [D5/16 B4]
    See all Cytokeratin 5 + 6 primary antibodies
  • Description
    Mouse monoclonal [D5/16 B4] to Cytokeratin 5 + 6
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Cytokeratin 5 (Human).

  • Positive control
    • Skin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberD5/16 B4
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • RelevanceCytokeratin 5 (58 kD) is a high molecular weight, basic type of cytokeratin expressed in basal, the intermediate and the superficial cell layers of stratified epithelia as well as transitional epithelia, complex epithelia and in mesothelial cells and mesothelioma. Cytokeratin 6 (56 kD) is also a high molecular weight, basic type cytokeratin expressed by proliferating squamous epithelium often paired with Cytokeratin 16.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • Cytokeratin 5 antibody
    • Cytokeratin 6A antibody
    • Cytokeratin 6B antibody
    • DDD antibody
    • EBS2 antibody
    • K5 antibody
    • K6A antibody
    • K6B antibody
    • Keratin 5 antibody
    • Keratin 6A antibody
    • Keratin 6B antibody
    • Keratin type II cytoskeletal 5 antibody
    • KRT 5 antibody
    • KRT 5A antibody
    • KRT 6A antibody
    • KRT 6B antibody
    see all

References for Anti-Cytokeratin 5 + 6 antibody [D5/16 B4] (ab17133)

This product has been referenced in:
  • Wen YH  et al. Id4 protein is highly expressed in triple-negative breast carcinomas: possible implications for BRCA1 downregulation. Breast Cancer Res Treat 135:93-102 (2012). IHC-P ; Human . Read more (PubMed: 22538771) »
  • Kurzen H  et al. Functional characterization of the epidermal cholinergic system in vitro. J Invest Dermatol 126:2458-72 (2006). Read more (PubMed: 16810300) »

See all 2 Publications for this product

Product Wall

Merci de nous avoir contactés.

L'anticorps ab17133 a une concentration de 16µg/ml.

Veuillez noter que le tampon de stockage contient 0.05% d'azoture de sodium et 1% de BSA, 2 éléments qui peuvent poser probl...

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DISCOUNT CODE:100%ABR-EXXXXX
Expiration date: 15/12/2012
I am very pleased to hear you would like to accept our offer and test ab17133 in Mouse. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer...

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Thank you for your enquiry and your interest in our products.
I have the confirmation from the Lab that this antibody has been validated only with human tissue specimens and we do not have any cross reactive with mouse samples.
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As I am sure you are aware, the antibody purchased, Anti-Cytokeratin 5 + 6 antibody (ab17133) was ordere...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1.5%
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Verified customer

Submitted Apr 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"