The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes IHC-P: Prediluted and ready to use. We suggest an incubation of 30 minutes. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Prediluted and ready to use on acetone fixed cryostat sections. We suggest an incubation of 30 minutes.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceCytokeratin 5 (58 kD) is a high molecular weight, basic type of cytokeratin expressed in basal, the intermediate and the superficial cell layers of stratified epithelia as well as transitional epithelia, complex epithelia and in mesothelial cells and mesothelioma. Cytokeratin 6 (56 kD) is also a high molecular weight, basic type cytokeratin expressed by proliferating squamous epithelium often paired with Cytokeratin 16.