Overview

  • Product name
    Anti-Cytokeratin 5 antibody
    See all Cytokeratin 5 primary antibodies
  • Description
    Rabbit polyclonal to Cytokeratin 5
  • Specificity
    ab53121 detects endogenous levels of total Cytokeratin 5 protein.
  • Tested applications
    Suitable for: ELISA, IHC-P, Flow Cyt, ICC/IF, IHC-FoFr, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    A synthetic peptide derived from human Cytokeratin 5.

  • Positive control
    • HepG2 cell extracts and human breast carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab53121 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 24648397ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 1 µg/ml.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21278345
IHC-Fr Use at an assay dependent concentration. PubMed: 22308455
WB 1/300 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).

Target

  • Involvement in disease
    Defects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
    Defects in KRT5 are the cause of epidermolysis bullosa simplex with migratory circinate erythema (EBSMCE) [MIM:609352]. EBSMCE is a form of intraepidermal epidermolysis bullosa characterized by unusual migratory circinate erythema. Skin lesions appear from birth primarily on the hands, feet, and legs but spare nails, ocular epithelia and mucosae. Lesions heal with brown pigmentation but no scarring. Electron microscopy findings are distinct from those seen in the DM-EBS, with no evidence of tonofilament clumping.
    Defects in KRT5 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
    Defects in KRT5 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, althought it is less severe.
    Defects in KRT5 are the cause of epidermolysis bullosa simplex with mottled pigmentation (MP-EBS) [MIM:131960]. MP-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering at acral sites and 'mottled' pigmentation of the trunk and proximal extremities with hyper- and hypopigmentation macules.
    Defects in KRT5 are the cause of Dowling-Degos disease (DDD) [MIM:179850]; also known as Dowling-Degos-Kitamura disease or reticulate acropigmentation of Kitamura. DDD is an autosomal dominant genodermatosis. Affected individuals develop a postpubertal reticulate hyperpigmentation that is progressive and disfiguring, and small hyperkeratotic dark brown papules that affect mainly the flexures and great skin folds. Patients usually show no abnormalities of the hair or nails.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 58 kDa cytokeratin antibody
    • CK-5 antibody
    • CK5 antibody
    • Cytokeratin-5 antibody
    • Cytokeratin5 antibody
    • DDD antibody
    • DDD1 antibody
    • EBS2 antibody
    • epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody
    • K2C5_HUMAN antibody
    • K5 antibody
    • keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) antibody
    • Keratin 5 antibody
    • Keratin antibody
    • keratin complex 2, basic, gene 5 antibody
    • keratin, type II cytoskeletal 5 antibody
    • Keratin-5 antibody
    • Keratin5 antibody
    • KRT 5 antibody
    • Krt5 antibody
    • KRT5A antibody
    • type II cytoskeletal 5 antibody
    • Type-II keratin Kb5 antibody
    see all

Images

  • ab53121 at 1/50 dilution staining Cytokeratin 5 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.ab53121 at 1/50 dilution staining Cytokeratin 5 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
  • ICC/IF image of ab53121 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53121, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Cytokeratin 5 antibody (ab53121) at 1/300 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HepG2 cell extract with immunising peptide


    Predicted band size : 62 kDa
    Observed band size : 62 kDa

References

This product has been referenced in:
  • Furukawa S  et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Rabbit . Read more (PubMed: 28190929) »
  • Dorà NJ  et al. Analysis of compound heterozygotes reveals that the mouse floxed Pax6 (tm1Ued) allele produces abnormal eye phenotypes. Transgenic Res 25:679-92 (2016). IHC ; Mouse . Read more (PubMed: 27240603) »

See all 25 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3)
Specification
NIH3T3
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Mar 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat airway epithelium)
Specification
Rat airway epithelium
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Nov 06 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (COS1)
Specification
COS1
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Ureter)
Specification
Ureter
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citric Acid Buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jun 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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