RabMAb

Anti-Cytokeratin 5 antibody [SP27] (ab64081)

Overview

  • Product nameAnti-Cytokeratin 5 antibody [SP27]
    See all Cytokeratin 5 primary antibodies
  • Description
    Rabbit monoclonal [SP27] to Cytokeratin 5
  • Tested applicationsWB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptides from C terminus of human keratin 5 protein.

  • Positive control
    • Human mesothelioma, A431 cell Lsates

Properties

Applications

Our Abpromise guarantee covers the use of ab64081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
Flow Cyt Use at an assay dependent concentration.
IHC-P 1/100. Incubate for 30 min at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

Target

  • Involvement in diseaseDefects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
    Defects in KRT5 are the cause of epidermolysis bullosa simplex with migratory circinate erythema (EBSMCE) [MIM:609352]. EBSMCE is a form of intraepidermal epidermolysis bullosa characterized by unusual migratory circinate erythema. Skin lesions appear from birth primarily on the hands, feet, and legs but spare nails, ocular epithelia and mucosae. Lesions heal with brown pigmentation but no scarring. Electron microscopy findings are distinct from those seen in the DM-EBS, with no evidence of tonofilament clumping.
    Defects in KRT5 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
    Defects in KRT5 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, althought it is less severe.
    Defects in KRT5 are the cause of epidermolysis bullosa simplex with mottled pigmentation (MP-EBS) [MIM:131960]. MP-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering at acral sites and 'mottled' pigmentation of the trunk and proximal extremities with hyper- and hypopigmentation macules.
    Defects in KRT5 are the cause of Dowling-Degos disease (DDD) [MIM:179850]; also known as Dowling-Degos-Kitamura disease or reticulate acropigmentation of Kitamura. DDD is an autosomal dominant genodermatosis. Affected individuals develop a postpubertal reticulate hyperpigmentation that is progressive and disfiguring, and small hyperkeratotic dark brown papules that affect mainly the flexures and great skin folds. Patients usually show no abnormalities of the hair or nails.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 58 kDa cytokeratin antibody
    • CK 5 antibody
    • CK-5 antibody
    • CK5 antibody
    • Cytokeratin-5 antibody
    • Cytokeratin5 antibody
    • DDD antibody
    • DDD1 antibody
    • EBS 2 antibody
    • EBS2 antibody
    • epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody
    • K2C5_HUMAN antibody
    • K5 antibody
    • Keratin 5 (epidermolysis bullosa simplex Dowling Meara/Kobner/Weber Cockayne types) antibody
    • keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) antibody
    • Keratin 5 antibody
    • Keratin antibody
    • keratin complex 2, basic, gene 5 antibody
    • Keratin Type II Cytoskeletal 5 antibody
    • keratin, type II cytoskeletal 5 antibody
    • Keratin-5 antibody
    • Keratin5 antibody
    • KRT 5 antibody
    • KRT 5A antibody
    • KRT5 antibody
    • KRT5A antibody
    • type II cytoskeletal 5 antibody
    • Type-II keratin Kb5 antibody
    see all

Anti-Cytokeratin 5 antibody [SP27] images

  • Flow cytometric analysis of rabbit anti-Cytoeratin 5 (SP27) antibody ab64081 (1/100) in A431 cells (green) compare to negative control of rabbit IgG (blue).

  • Immunohistochemical analysis of Cytokeratin 5 expression in formalin fixed paraffin embedded human mesothelioma tissue section using ab64081 at 1/100 dlution.
  • Anti-Cytokeratin 5 antibody [SP27] (ab64081) at 1/100 dilution + A431 cell lysate

    Predicted band size : 62 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)

References for Anti-Cytokeratin 5 antibody [SP27] (ab64081)

ab64081 has not yet been referenced specifically in any publications.

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