Recombinant Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1602Y] to Cytokeratin 6
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cytokeratin 6 antibody [EPR1602Y]
See all Cytokeratin 6 primary antibodies -
Description
Rabbit monoclonal [EPR1602Y] to Cytokeratin 6 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Cytokeratin 6 aa 1-100 (N terminal). The exact sequence is proprietary. P48668, P04259 and P02538 100% homology.
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Positive control
- WB: HaCaT and A431 cell lysates, human skin and tonsil tissue lysates. IHC-P: Human tonsil and squamous cervical carcinoma tissues. Human normal skin tissue. ICC/IF: A431 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1602Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/2000. Predicted molecular weight: 60 kDa.
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ICC/IF |
1/50.
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Notes |
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IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/2000. Predicted molecular weight: 60 kDa. |
ICC/IF
1/50. |
Target
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Tissue specificity
Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath. -
Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 286887 Human
- Entrez Gene: 3853 Human
- Entrez Gene: 3854 Human
- Omim: 148041 Human
- Omim: 148042 Human
- Omim: 612315 Human
- SwissProt: P02538 Human
- SwissProt: P04259 Human
see all -
Alternative names
- CK 6A antibody
- CK 6B antibody
- CK 6C antibody
see all
Images
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IHC image of Cytokeratin 6 staining in a section of formalin-fixed paraffin-embedded normal human skin* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab93279, 0.5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre -
All lanes : Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279) at 1/1000 dilution
Lane 1 : N-GST tagged full-length recombinant human Cytokeratin 6A protein, 10 ng
Lane 2 : N-GST tagged full-length recombinant human Cytokeratin 5 protein, 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking buffer: 5% NFDM /TBST.
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All lanes : Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279) at 1/2000 dilution (purified)
Lane 1 : Human skin tissue lysate
Lane 2 : Human tonsil tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 60 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Cytokeratin 6 with purified ab93279 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).
Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunocytochemistry/Immunofluorescence analysis of A431 (Human epidermoid carcinoma cell line) cells labelling Cytokeratin 6 with purified ab93279 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.
Control 1: Primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
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ICC/IF image of unpurified ab93279 stained A431 (Human epidermoid carcinoma cell line) cells.
The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93279, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabiit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279) at 1/10000 dilution (purified) + HaCaT (Human keratinocyte cell line) cell lysate at 20 µg
Secondary
Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 60 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279) at 1/5000 dilution (unpurified) + A431 (Human epidermoid carcinoma cell line) cell lysate at 10 µg
Predicted band size: 60 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human squamous cervical carcinoma tissue labelling Cytokeratin 6 with unpurified ab93279 at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab93279 has been referenced in 8 publications.
- Wang J et al. The Circular RNA CircCOL1A1 Functions as a miR-149-5p Sponge to Regulate the Formation of Superior-Quality Brush Hair via the CMTM3/AR Axis. Front Cell Dev Biol 10:760466 (2022). PubMed: 35186916
- Moreira KG et al. Accelerative action of topical piperonylic acid on mice full thickness wound by modulating inflammation and collagen deposition. PLoS One 16:e0259134 (2021). PubMed: 34699564
- Su Y et al. Pre-aggregation of scalp progenitor dermal and epidermal stem cells activates the WNT pathway and promotes hair follicle formation in in vitro and in vivo systems. Stem Cell Res Ther 10:403 (2019). PubMed: 31856904
- Parfitt GJ Immunofluorescence Tomography: High-resolution 3-D reconstruction by serial-sectioning of methacrylate embedded tissues and alignment of 2-D immunofluorescence images. Sci Rep 9:1992 (2019). PubMed: 30760855
- Kuo WL et al. Establishment of two basal-like breast cancer cell lines with extremely low tumorigenicity from Taiwanese premenopausal women. Hum Cell 31:154-166 (2018). PubMed: 29484537
- Press E et al. Disease-linked connexin26 S17F promotes volar skin abnormalities and mild wound healing defects in mice. Cell Death Dis 8:e2845 (2017). PubMed: 28569788
- Hampel U et al. Serum-induced keratinization processes in an immortalized human meibomian gland epithelial cell line. PLoS One 10:e0128096 (2015). PubMed: 26042605
- Rice RH et al. Distinguishing ichthyoses by protein profiling. PLoS One 8:e75355 (2013). WB ; Human . PubMed: 24130705