The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 22679510
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
FunctionBlocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Tissue specificityExpressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
Sequence similaritiesBelongs to the intermediate filament family.
Post-translational modificationsArg-20 is dimethylated, probably to asymmetric dimethylarginine.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [LP1K] (ab20206)Image from Zhang Q et al., PLoS One. 2012;7(6):e38487. Epub 2012 Jun 5. Fig 1.; doi:10.1371/journal.pone.0038487; June 5, 2012, PLoS ONE 7(6): e38487.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placental villi in the first trimester. Cytokeratin 7 was stained using ab20206 at 5 µg/ml. Sections were incubated with biotinylated secondary antibody followed by streptavidin-peroxidase, and was detected with DAB.
References for Anti-Cytokeratin 7 antibody [LP1K] (ab20206)