For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Cytokeratins from the human bladder carcinoma cell line T24.
Our Abpromise guarantee covers the use of ab9021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/100 - 1/200.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||1/100 - 1/500.|
|ICC||1/100 - 1/500.|
|WB||1/100 - 1/1000.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 19233130|
ab9021 staining normal breast tissue section (frozen), fixated in Methanol, staining epithelial cells. These pictures are part of the review submitted by Rene Villadsen.
ab9021 staining Cytokeratin 7 in mouse liver, heterogeneous primary cell culture by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 3% Mouse Ig Blocking reagent (component of a commercially available mouse on mouse MOM kit) at 200C for 30 minutes. Samples were incubated with primary antibody (1/100: in dilution buffer from MOM kit) for 1 hour at 200C. An Alexa Fluor ® 568-conjugated rabbit polyclonal to mouse IgG was used as secondary antibody at 1/250 dilution.
Image courtesy of an anonymous Abreview.Blocked with 3% BSA for 2 hours.