Sheep, Cow, Dog, Human, Pig Does not react with:
Mouse, Rat, Rabbit
Tissue/ cell preparation (Human). (Cytoskeleton preparation of HeLa cells (cervix carcinoma)).
General notesStrongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC: Use at an assay dependant concentration.
IHC-P: Use at an assay dependant dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IP: Use at an assay dependant dilution.
WB: Use at an assay dependant concentration.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceCytokeratin 7 and 17 is a heterotetramer of a type I and type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7. Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Cytokeratin 17 is required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling.
Ab670 (20ug/ml) staining human Cytokeratin 7+17 in human pancreas by immunohistochemistry using formalin fixed, paraffin embedded tissue.
References for Anti-Cytokeratin 7+17 antibody [C-46] (ab670)
This product has been referenced in:
Sun Y et al. Aberrant cytokeratin expression during arsenic-induced acquired malignant phenotype in human HaCaT keratinocytes consistent with epidermal carcinogenesis. Toxicology262:162-70 (2009).
Read more (PubMed: 19524636) »
Vojtesek B et al. A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis. Neoplasma37:333-42 (1990).
Read more (PubMed: 1695327) »