• Product nameAnti-Cytokeratin 8 antibody [35bH11]
    See all Cytokeratin 8 primary antibodies
  • Description
    Mouse monoclonal [35bH11] to Cytokeratin 8
  • SpecificityThis antibody recognizes a keratin polypeptide of 54 kDa. It stains strongly with non-squamous epithelium but does not stain squamous epithelium. It reacts with the sweat gland and ducts in the skin. Adenocarcinoma of the ovary, gastrointestinal tract, ductal carcinoma of breast, pancreas and bile ducts stain positive with this antibody.
  • Tested applicationsSuitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B



Our Abpromise guarantee covers the use of ab758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIHC: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method.
    Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
    Enzymatic antigen retrieval of paraffin embedded tissues is recommended prior to the immunostaining.
  • Target

    • FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
    • Involvement in diseaseCirrhosis
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Post-translational
      Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
      O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
    • Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CARD2 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK8 antibody
      • CYK8 antibody
      • CYKER antibody
      • Cytokeratin endo A antibody
      • Cytokeratin-8 antibody
      • DreK8 antibody
      • EndoA antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 8 antibody
      • Keratin type II cytoskeletal 8 antibody
      • Keratin, type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • Krt 2.8 antibody
      • KRT8 antibody
      • MGC118110 antibody
      • MGC174782 antibody
      • MGC53564 antibody
      • MGC85764 antibody
      • sb:cb186 antibody
      • Type-II keratin Kb8 antibody
      see all

    Anti-Cytokeratin 8 antibody [35bH11] images

    • ab758 - immunohistochemistry

      Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 8, using ABC and DAB chromagen.

    • ab758 staining Cytokeratin 8 in formalin-fixed, paraffin-embedded Human colon tissue by Immunohistochemistry. Staining was detected using DAB.

    References for Anti-Cytokeratin 8 antibody [35bH11] (ab758)

    This product has been referenced in:
    • Berger R  et al. Androgen-induced differentiation and tumorigenicity of human prostate epithelial cells. Cancer Res 64:8867-75 (2004). Read more (PubMed: 15604246) »

    See 1 Publication for this product

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    For ab758, the immunogen that was used was cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B. We have not validated this antibody in pig. But the human sequence is 85% homologous with the pig ...

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