Anti-Cytokeratin 8 antibody (ab51152)

Overview

  • Product nameAnti-Cytokeratin 8 antibody
    See all Cytokeratin 8 primary antibodies
  • Description
    Rabbit polyclonal to Cytokeratin 8
  • SpecificityKeratin 8 (Ab-73) antibody detects endogenous levels of total Keratin 8 protein.
  • Tested applicationsSuitable for: Indirect ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic non-phosphopeptide derived from human Keratin 8 around the phosphorylation site of serine 73 (L-L-SP-P-L).

  • Positive control
    • Human breast carcinoma tissue, HeLa cell extract treated with Anisomycin at 25µg/ml for 30 minutes. IF/ICC: MCF7 cell line.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab51152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 54 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
  • Involvement in diseaseCirrhosis
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CARD2 antibody
    • CK 8 antibody
    • CK-8 antibody
    • CK8 antibody
    • CYK8 antibody
    • CYKER antibody
    • Cytokeratin endo A antibody
    • Cytokeratin-8 antibody
    • DreK8 antibody
    • EndoA antibody
    • K2C8 antibody
    • K2C8_HUMAN antibody
    • K8 antibody
    • Keratin 8 antibody
    • Keratin type II cytoskeletal 8 antibody
    • Keratin, type II cytoskeletal 8 antibody
    • Keratin-8 antibody
    • KO antibody
    • Krt 2.8 antibody
    • KRT8 antibody
    • MGC118110 antibody
    • MGC174782 antibody
    • MGC53564 antibody
    • MGC85764 antibody
    • sb:cb186 antibody
    • Type-II keratin Kb8 antibody
    see all

Anti-Cytokeratin 8 antibody images

  • ICC/IF image of ab51152 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51152, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • This image shows human breast carcinoma tissue stained with ab51152 at 1/50 dilution. The cells in the right hand image were treated with the immunising peptide, the cells in the left hand image were not.

References for Anti-Cytokeratin 8 antibody (ab51152)

ab51152 has not yet been referenced specifically in any publications.

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La séquence exacte du peptide utilisécomme immunogène est confidentielle.
L'anticorps polyclonalab51152 reconnait spécifiquement les formes phosphorylées et non-phos...

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Les dilutions recommandées pour ab52949 sont :
Western Blot : 1/500
IHC-P et ICC/IF : 1/100 - 1/250
IP : 1/60

Pour l'anticorps ab51152, la concentration est de 1mg/ml. L'immunogèneayant servi à produire cet...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"