Anti-Cytokeratin 8 antibody (ab52949)


  • Product nameAnti-Cytokeratin 8 antibody
    See all Cytokeratin 8 primary antibodies
  • Description
    Rabbit polyclonal to Cytokeratin 8
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    A synthetic peptide corresponding to residues on the N-terminus of human Cytokeratin 8

  • Positive control
    • HeLa cell lysate, human gastric adenocarcinoma and HeLa cells.


Associated products


Our Abpromise guarantee covers the use of ab52949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IP 1/60.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
    • Involvement in diseaseCirrhosis
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Post-translational
      Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
      O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
    • Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CARD2 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK8 antibody
      • CYK8 antibody
      • CYKER antibody
      • Cytokeratin endo A antibody
      • Cytokeratin-8 antibody
      • DreK8 antibody
      • EndoA antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 8 antibody
      • Keratin type II cytoskeletal 8 antibody
      • Keratin, type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • Krt 2.8 antibody
      • KRT8 antibody
      • MGC118110 antibody
      • MGC174782 antibody
      • MGC53564 antibody
      • MGC85764 antibody
      • sb:cb186 antibody
      • Type-II keratin Kb8 antibody
      see all

    Anti-Cytokeratin 8 antibody images

    • Anti-Cytokeratin 8 antibody (ab52949) at 1/500 dilution + HeLa cell lysate at 10 µg

      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 54 kDa
      Observed band size : 54 kDa
    • ab52949 at 1/100 dilution staining Cytokeratin 8 in human gastric adenocarcinoma by Immunohistochemistry, Paraffin embedded tissue.
    • ab52949 at 1/100 dilution staining Cytokeratin 8 in HeLa cells by Immunofluorescence.

    References for Anti-Cytokeratin 8 antibody (ab52949)

    ab52949 has not yet been referenced specifically in any publications.

    Product Wall

    Les dilutions recommandées pour ab52949 sont :
    Western Blot : 1/500
    IHC-P et ICC/IF : 1/100 - 1/250
    IP : 1/60

    Pour l'anticorps ab51152, la concentration est de 1mg/ml. L'immunogèneayant servi à produire cet...

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    Je vous confirme que l'anticorpsab52949 est dirigé contre la partie N-terminale de la cytokeratine 8 humaine.