Overview

  • Product nameAnti-Cytokeratin 8 antibody
    See all Cytokeratin 8 primary antibodies
  • Description
    Rabbit polyclonal to Cytokeratin 8
  • Specificityab53708 detects endogenous levels of total Cytokeratin 8 protein.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from human Cytokeratin 8.

  • Positive control
    • SKOV3 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab53708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/40000.

Target

  • FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
  • Involvement in diseaseCirrhosis
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CARD2 antibody
    • CK 8 antibody
    • CK-8 antibody
    • CK8 antibody
    • CYK8 antibody
    • CYKER antibody
    • Cytokeratin endo A antibody
    • Cytokeratin-8 antibody
    • DreK8 antibody
    • EndoA antibody
    • K2C8 antibody
    • K2C8_HUMAN antibody
    • K8 antibody
    • Keratin 8 antibody
    • Keratin type II cytoskeletal 8 antibody
    • Keratin, type II cytoskeletal 8 antibody
    • Keratin-8 antibody
    • KO antibody
    • Krt 2.8 antibody
    • KRT8 antibody
    • MGC118110 antibody
    • MGC174782 antibody
    • MGC53564 antibody
    • MGC85764 antibody
    • sb:cb186 antibody
    • Type-II keratin Kb8 antibody
    see all

Anti-Cytokeratin 8 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue section labeling Cytokeratin 8 with ab53708. The picture on the right is blocked with the synthesized peptide.

  • All lanes : Anti-Cytokeratin 8 antibody (ab53708) at 1/500 dilution

    Lane 1 : SKOV3 cell extract
    Lane 2 : SKOV3 cell extract with immunising peptide


    Predicted band size : 54 kDa
    Observed band size : 54 kDa

References for Anti-Cytokeratin 8 antibody (ab53708)

This product has been referenced in:
  • Ding Y  et al. Proteomic analysis of colonic mucosa in a rat model of irritable bowel syndrome. Proteomics 10:2620-2630 (2010). WB ; Rat . Read more (PubMed: 20461717) »
  • Yocum AK  et al. Coupled global and targeted proteomics of human embryonic stem cells during induced differentiation. Mol Cell Proteomics 7:750-67 (2008). Read more (PubMed: 18304949) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"