Anti-Cytokeratin 8 antibody [SB37b] (FITC) (ab87010)

Overview

  • Product name
    Anti-Cytokeratin 8 antibody [SB37b] (FITC)
    See all Cytokeratin 8 primary antibodies
  • Description
    Mouse monoclonal [SB37b] to Cytokeratin 8 (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    ab87010 reacts specifically with cytokeratin 8 and the recombinant C terminal of cytokeratin 8.
  • Tested applications
    Suitable for: ICC/IF, ELISA, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant C terminal fragment of Cytokeratin 8

  • Positive control
    • HT29 and HCC38 cells. HT29 and HCC38 cell lysates.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab87010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 54 kDa.
IHC-Fr Use a concentration of 10 µg/ml.

Target

  • Function
    Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificity
    Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
  • Involvement in disease
    Cirrhosis
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • Cellular localization
    Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CARD2 antibody
    • CK 8 antibody
    • CK-8 antibody
    • CK8 antibody
    • CYK8 antibody
    • CYKER antibody
    • Cytokeratin endo A antibody
    • Cytokeratin-8 antibody
    • DreK8 antibody
    • EndoA antibody
    • K2C8 antibody
    • K2C8_HUMAN antibody
    • K8 antibody
    • Keratin 8 antibody
    • Keratin type II cytoskeletal 8 antibody
    • Keratin, type II cytoskeletal 8 antibody
    • Keratin-8 antibody
    • KO antibody
    • Krt 2.8 antibody
    • KRT8 antibody
    • MGC118110 antibody
    • MGC174782 antibody
    • MGC53564 antibody
    • MGC85764 antibody
    • sb:cb186 antibody
    • Type-II keratin Kb8 antibody
    see all

Images

  • ab87010, at 10 µg/ml, staining Cytokeratin 8 in frozen sections of the HCC38 pancreatic carcinoma cell line by Immunohistochemistry. Positive cells are viewed by fluorescence microscopy.

References

ab87010 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


The source of the antibody considers the F:P ratio to be proprietary.
They have not measured the isoelectric point (pI) of this particular antibody, but some studies indicate that most mouse monoclonal antibodies fall in the range of 6.4 - 7.6...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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