• Product nameAnti-Cytokeratin 8 antibody [TS1]
    See all Cytokeratin 8 primary antibodies
  • Description
    Mouse monoclonal [TS1] to Cytokeratin 8
  • Specificityab49778 recognises cytokeratin 8 intermediate filament.
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified tumour-derived cytokeratins.

  • Positive control
    • IHC-P: Human lung tissue. ICC/IF: HeLa cells.


Associated products


Our Abpromise guarantee covers the use of ab49778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100 - 1/200.

Acetone fixation recommended.

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
    • Involvement in diseaseCirrhosis
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Post-translational
      Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
      O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
    • Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CARD2 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK8 antibody
      • CYK8 antibody
      • CYKER antibody
      • Cytokeratin endo A antibody
      • Cytokeratin-8 antibody
      • DreK8 antibody
      • EndoA antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 8 antibody
      • Keratin type II cytoskeletal 8 antibody
      • Keratin, type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • Krt 2.8 antibody
      • KRT8 antibody
      • MGC118110 antibody
      • MGC174782 antibody
      • MGC53564 antibody
      • MGC85764 antibody
      • sb:cb186 antibody
      • Type-II keratin Kb8 antibody
      see all

    Anti-Cytokeratin 8 antibody [TS1] images

    • ICC/IF image of ab49778 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49778, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG(H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

    • Immunostaining of human lung tissue with ab49778.

    References for Anti-Cytokeratin 8 antibody [TS1] (ab49778)

    This product has been referenced in:
    • Holm P  et al. Functional mapping and single chain construction of the anti-cytokeratin 8 monoclonal antibody TS1. Mol Immunol 44:1075-84 (2007). Read more (PubMed: 16989900) »
    • Erlandsson A  et al. In vivo clearing of idiotypic antibodies with antiidiotypic antibodies and their derivatives. Mol Immunol 43:599-606 (2006). Read more (PubMed: 15978666) »

    See all 4 Publications for this product

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