1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/250.
FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Involvement in diseaseCirrhosis
Sequence similaritiesBelongs to the intermediate filament family.
Post-translational modificationsPhosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
ab76584, at a 1/100 dilution, staining Cytokeratin 8 in paraffin embedded human gastric adenocarcinoma (image 1) and human breast adenocarcinoma (image 2) tissues by Immunohistochemistry.
Western blot - Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584)
All lanes : Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584) at 1/50000 dilution
Lane 1 : HeLa cell lysate, untreated Lane 2 : HeLa cell lysate treated with Calyculin
Lysates/proteins at 10 µg per lane.
Secondary HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 54 kDa Observed band size : 54 kDa
References for Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584)
This product has been referenced in:
Kakehashi A et al. Cytokeratin 8/18 overexpression and complex formation as an indicator of GST-P positive foci transformation into hepatocellular carcinomas. Toxicol Appl Pharmacol238:71-9 (2009).
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