Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584)

Overview

  • Product nameAnti-Cytokeratin 8 (phospho S23) antibody [EP1629Y]
    See all Cytokeratin 8 primary antibodies
  • Description
    Rabbit monoclonal [EP1629Y] to Cytokeratin 8 (phospho S23)
  • Specificityab76584 detects Cytokeratin 8 phosphorylated on serine 23.
  • Tested applicationsSuitable for: IHC-Fr, IP, WB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding serine 23 of human Cytokeratin 8

  • Positive control
    • HeLa cell lysates, untreated or treated with Calyculin; human gastric adenocarcinoma and human breast adenocarcinoma tissues.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 19409407
IP 1/10 - 1/100.
WB 1/10000 - 1/50000. Predicted molecular weight: 54 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.

Target

  • FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
  • Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
  • Involvement in diseaseCirrhosis
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
    O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CARD2 antibody
    • CK 8 antibody
    • CK-8 antibody
    • CK8 antibody
    • CYK8 antibody
    • CYKER antibody
    • Cytokeratin endo A antibody
    • Cytokeratin-8 antibody
    • DreK8 antibody
    • EndoA antibody
    • K2C8 antibody
    • K2C8_HUMAN antibody
    • K8 antibody
    • Keratin 8 antibody
    • Keratin type II cytoskeletal 8 antibody
    • Keratin, type II cytoskeletal 8 antibody
    • Keratin-8 antibody
    • KO antibody
    • Krt 2.8 antibody
    • KRT8 antibody
    • MGC118110 antibody
    • MGC174782 antibody
    • MGC53564 antibody
    • MGC85764 antibody
    • sb:cb186 antibody
    • Type-II keratin Kb8 antibody
    see all

Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] images

  • ab76584, at a 1/100 dilution, staining Cytokeratin 8 in paraffin embedded human gastric adenocarcinoma (image 1) and human breast adenocarcinoma (image 2) tissues by Immunohistochemistry.
  • All lanes : Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584) at 1/50000 dilution

    Lane 1 : HeLa cell lysate, untreated
    Lane 2 : HeLa cell lysate treated with Calyculin

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 54 kDa
    Observed band size : 54 kDa

References for Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584)

This product has been referenced in:
  • Kakehashi A  et al. Cytokeratin 8/18 overexpression and complex formation as an indicator of GST-P positive foci transformation into hepatocellular carcinomas. Toxicol Appl Pharmacol 238:71-9 (2009). IHC-Fr . Read more (PubMed: 19409407) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"