Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
1/50 - 1/100.
FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Involvement in diseaseCirrhosis
Sequence similaritiesBelongs to the intermediate filament family.
Post-translational modificationsPhosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue stained with ab59434 at 1/50 dilution; in the presence or absence of the phosphopeptide.
Western blot - Cytokeratin 8 (phospho S431) antibody (ab59434)
All lanes : Anti-Cytokeratin 8 (phospho S431) antibody (ab59434) at 1/500 dilution
Lane 1 : 293 cell extract treated with EGF (200ng/ml, 30 mins) Lane 2 : 293 cell extract treated with EGF (200ng/ml, 30 mins) with phosphopeptide
Predicted band size : 54 kDa Observed band size : 54 kDa
References for Anti-Cytokeratin 8 (phospho S431) antibody (ab59434)
This product has been referenced in:
Khapare N et al. Plakophilin3 Loss Leads to an Increase in PRL3 Levels Promoting K8 Dephosphorylation, Which Is Required for Transformation and Metastasis. PLoS One7:e38561 (2012).
Read more (PubMed: 22701666) »
Alam H et al. Loss of Keratin 8 Phosphorylation Leads to Increased Tumor Progression and Correlates with Clinico-Pathological Parameters of OSCC Patients. PLoS One6:e27767 (2011).
Read more (PubMed: 22114688) »