Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)


  • Product nameAnti-Cytokeratin 8 (phospho S431) antibody [EP1630]
    See all Cytokeratin 8 primary antibodies
  • Description
    Rabbit monoclonal [EP1630] to Cytokeratin 8 (phospho S431)
  • Specificityab109452 only detects Cytokeratin 8 phosphorylated at Serine 431
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues surrounding Serine 431 of Human Cytokeratin 8.

  • Positive control
    • HeLa lysates, treated with FBS and Calyculin; Human ductal breast carcinoma and Human stomach adenocarcinoma tissues.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab109452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 54 kDa).
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
    • Involvement in diseaseCirrhosis
    • Sequence similaritiesBelongs to the intermediate filament family.
    • Post-translational
      Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
      O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
    • Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CARD2 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK8 antibody
      • CYK8 antibody
      • CYKER antibody
      • Cytokeratin endo A antibody
      • Cytokeratin-8 antibody
      • DreK8 antibody
      • EndoA antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 8 antibody
      • Keratin type II cytoskeletal 8 antibody
      • Keratin, type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • Krt 2.8 antibody
      • KRT8 antibody
      • MGC118110 antibody
      • MGC174782 antibody
      • MGC53564 antibody
      • MGC85764 antibody
      • sb:cb186 antibody
      • Type-II keratin Kb8 antibody
      see all

    Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] images

    • All lanes : Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452) at 1/10000 dilution

      Lane 1 : HeLa lysates, untreated
      Lane 2 : HeLa lysates, treated with FBS and Calyculin A

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 54 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    • ab109452 at 1/100 dilution staining Cytokeratin 8 in Human ductal breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109452 at 1/100 dilution staining Cytokeratin 8 in Human stomach adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    References for Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)

    This product has been referenced in:
    • Kuga T  et al. A novel mechanism of keratin cytoskeleton organization through casein kinase Ia and FAM83H in colorectal cancer. J Cell Sci 126:4721-31 (2013). Saccharomyces cerevisiae . Read more (PubMed: 23902688) »

    See 1 Publication for this product

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