Rabbit monoclonal [E431-2] to Cytokeratin 8 (phospho S73)
SpecificityThe antibody only detects Keratin 8 phosphorylated on Serine 73. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applicationsSuitable for:
IHC-Fr, WB, ICC/IF, IHC-Pmore details Unsuitable for:
Flow Cyt or IP
Mouse, Rat, Human
Full length native protein (purified) corresponding to Human Cytokeratin 8. Isolated from cells pretreated with okadaic acid or pervanadate to promote Serine hyperphosphorylation,
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 19409407
1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Use at an assay dependent concentration.
Application notesIs unsuitable for Flow Cyt or IP.
FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Involvement in diseaseCirrhosis
Sequence similaritiesBelongs to the intermediate filament family.
Post-translational modificationsPhosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
IHC-P analysis (with antigen retrieval) of liver carcinoma using anti-Phospho-Keratin 8 (ab32579) diluted 1/500.
Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 (phospho S73) antibody [E431-2] (ab32579)This image is courtesy of an Abreview submitted by Dr Hannah Whiteman
Ab32579 staining cells from a human pancreatic cell line by ICC/IF. The cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C. Ab32579 was diluted 1/25 and incubated with the sample for 1 hour at 22°C. An Alexa Fluor® 488 conjugated donkey anti-rabbit antibody was used as the secondary.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S73) antibody [E431-2] (ab32579)This image is courtesy of an anonymous Abreview
Immunohistochemical analysis of Mouse lung adenoma, staining Cytokeratin 8 (phospho S73) with ab32579.
Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/1000 in PBS) for 8 hours at 4°C. A biotin-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.