Anti-Cytokeratin 8 (phospho S73) antibody [E431-2] (ab32579)

Overview

  • Product name
    Anti-Cytokeratin 8 (phospho S73) antibody [E431-2]
    See all Cytokeratin 8 primary antibodies
  • Description
    Rabbit monoclonal [E431-2] to Cytokeratin 8 (phospho S73)
  • Specificity
    The antibody only detects Keratin 8 phosphorylated on Serine 73. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Cytokeratin 8. Isolated from cells pretreated with okadaic acid or pervanadate to promote Serine hyperphosphorylation,

  • Positive control
    • WB: A431 cell lysate ICC/IF: A431 cells IHC: liver carcinoma
  • General notes

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 19409407
WB 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF 1/250.
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
    • Tissue specificity
      Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
    • Involvement in disease
      Cirrhosis
    • Sequence similarities
      Belongs to the intermediate filament family.
    • Post-translational
      modifications
      Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
      O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
    • Cellular localization
      Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CARD2 antibody
      • CK 8 antibody
      • CK-8 antibody
      • CK8 antibody
      • CYK8 antibody
      • CYKER antibody
      • Cytokeratin endo A antibody
      • Cytokeratin-8 antibody
      • DreK8 antibody
      • EndoA antibody
      • K2C8 antibody
      • K2C8_HUMAN antibody
      • K8 antibody
      • Keratin 8 antibody
      • Keratin type II cytoskeletal 8 antibody
      • Keratin, type II cytoskeletal 8 antibody
      • Keratin-8 antibody
      • KO antibody
      • Krt 2.8 antibody
      • KRT8 antibody
      • MGC118110 antibody
      • MGC174782 antibody
      • MGC53564 antibody
      • MGC85764 antibody
      • sb:cb186 antibody
      • Type-II keratin Kb8 antibody
      see all

    Anti-Cytokeratin 8 (phospho S73) antibody [E431-2] images

    • Immunofluorescent staining of A431 cells using anti-Phospho-Keratin 8 (ab32579) diluted 1/250.
    • IHC-P analysis (with antigen retrieval) of liver carcinoma using anti-Phospho-Keratin 8 (ab32579) diluted 1/500.
    • Ab32579 staining cells from a human pancreatic cell line by ICC/IF.  The cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C.  Ab32579 was diluted 1/25 and incubated with the sample for 1 hour at 22°C.  An Alexa Fluor® 488 conjugated donkey anti-rabbit antibody was used as the secondary.

      See Abreview

    • Immunohistochemical analysis of Mouse lung adenoma, staining Cytokeratin 8 (phospho S73) with ab32579.

      Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/1000 in PBS) for 8 hours at 4°C. A biotin-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.

      See Abreview

    References for Anti-Cytokeratin 8 (phospho S73) antibody [E431-2] (ab32579)

    This product has been referenced in:
    • Corfe BM  et al. Inflammation decreases keratin level in ulcerative colitis; inadequate restoration associates with increased risk of colitis-associated cancer. BMJ Open Gastroenterol 2:e000024 (2015). WB . Read more (PubMed: 26462276) »
    • Kuga T  et al. A novel mechanism of keratin cytoskeleton organization through casein kinase Ia and FAM83H in colorectal cancer. J Cell Sci 126:4721-31 (2013). WB ; Human . Read more (PubMed: 23902688) »

    See all 6 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human cancer cell line A549)
    Loading amount
    25 µg
    Specification
    human cancer cell line A549
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
    Username

    Abcam user community

    Verified customer

    Submitted Sep 28 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (lung adenoma)
    Specification
    lung adenoma
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 25 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (lung adenoma)
    Specification
    lung adenoma
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 24 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (lung cancer primary tumor)
    Loading amount
    25 µg
    Specification
    lung cancer primary tumor
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 24 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (pancreatic cancer cell line)
    Loading amount
    20 µg
    Specification
    pancreatic cancer cell line
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 14 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Pancreatic cancer cell line)
    Specification
    Pancreatic cancer cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Username

    Dr. Hannah Whiteman

    Verified customer

    Submitted Jun 18 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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