Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)

Overview

  • Product nameAnti-Cytokeratin 8+18 antibody [5D3]
    See all Cytokeratin 8+18 primary antibodies
  • Description
    Mouse monoclonal [5D3] to Cytokeratin 8+18
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Cytokeratins from the human breast carcinoma cell line MCF-7.

  • Positive control
    • Skin
  • General notes


    This antibody stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, Tissue culture supernatant
  • PurityTissue culture supernatant
  • Primary antibody notesThis antibody stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.
  • ClonalityMonoclonal
  • Clone number5D3
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 21102523
IHC-P 1/25 - 1/50. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceFunction: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. Disease: Cirrhosis Similarity: Belongs to the intermediate filament family. PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • CARD2 antibody
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • CK 8 antibody
    • CK-8 antibody
    • CK18 antibody
    • CK8 antibody
    • CYK18 antibody
    • CYK8 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin 8 antibody
    • Cytokeratin-8 antibody
    • K18 antibody
    • K2C8 antibody
    • K2C8_HUMAN antibody
    • K8 antibody
    • Keratin 18 antibody
    • Keratin 8 antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 18 antibody
    • keratin type II cytoskeletal 8 antibody
    • Keratin-8 antibody
    • KO antibody
    • KRT18 antibody
    • KRT8 antibody
    • type II cytoskeletal 8 antibody
    • Type-II keratin Kb8 antibody
    see all

Anti-Cytokeratin 8+18 antibody [5D3] images

  • ab17139 staining human mammacarcinoma by IHC-P.
  • Overlay histogram showing MCF7 cells stained with ab17139 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17139, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • ab17139 staining Cytokeratin 8+18 in MCF7 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 1 hour at room temperature. The primary antibody was diluted 1/1000 and incubated with the sample for 1 hour at room temperature. The secondary antibody was Alexa Fluor 488®-conjugated Goat anti-Mouse, diluted 1/1000.

    See Abreview

References for Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)

This product has been referenced in:
  • Wang Y  et al. Transforming growth factor-ß regulates the sphere-initiating stem cell-like feature in breast cancer through miRNA-181 and ATM. Oncogene : (2010). ICC/IF ; Human . Read more (PubMed: 21102523) »

See 1 Publication for this product

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I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any Cytokeratin 8+18 antibody- an...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (MCF7 Breast Cancer Cells)
Specification MCF7 Breast Cancer Cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
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Dr. Sharon Sneddon

Verified customer

Submitted Nov 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"