Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)


  • Product nameAnti-Cytokeratin 8+18 antibody [5D3]
    See all Cytokeratin 8+18 primary antibodies
  • Description
    Mouse monoclonal [5D3] to Cytokeratin 8+18
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Cytokeratins from the human breast carcinoma cell line MCF-7.

  • Positive control
    • Skin
  • General notes

    This antibody stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, Tissue culture supernatant
  • PurityTissue culture supernatant
  • Primary antibody notesThis antibody stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.
  • ClonalityMonoclonal
  • Clone number5D3
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab17139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 21102523
IHC-P 1/25 - 1/50. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • RelevanceCytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with Cytokeratin 18 (type A; acidic subfamily of low molecular weight keratins).
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • CARD2 antibody
    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • CK 8 antibody
    • CK18 antibody
    • CK8 antibody
    • CYK18 antibody
    • CYK8 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin 8 antibody
    • K18 antibody
    • K2C8 antibody
    • K8 antibody
    • Keratin 18 antibody
    • Keratin 8 antibody
    • Keratin type I cytoskeletal 18 antibody
    • keratin type II cytoskeletal 8 antibody
    • KO antibody
    • KRT18 antibody
    • KRT8 antibody
    see all

Anti-Cytokeratin 8+18 antibody [5D3] images

  • ab17139 staining human mammacarcinoma by IHC-P.
  • Overlay histogram showing MCF7 cells stained with ab17139 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17139, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • ab17139 staining Cytokeratin 8+18 in MCF7 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 1 hour at room temperature. The primary antibody was diluted 1/1000 and incubated with the sample for 1 hour at room temperature. The secondary antibody was Alexa Fluor 488®-conjugated Goat anti-Mouse, diluted 1/1000.

    See Abreview

References for Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)

This product has been referenced in:
  • Wang Y  et al. Transforming growth factor-ß regulates the sphere-initiating stem cell-like feature in breast cancer through miRNA-181 and ATM. Oncogene : (2010). ICC/IF ; Human . Read more (PubMed: 21102523) »

See 1 Publication for this product

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Thank you for your enquiry.

I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any Cytokeratin 8+18 antibody- an...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (MCF7 Breast Cancer Cells)
Specification MCF7 Breast Cancer Cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Dr. Sharon Sneddon

Verified customer

Submitted Nov 30 2011