Mouse monoclonal [5D3] to Cytokeratin 8+18, prediluted
SpecificityThis antibody is specific to human cytokeratins 8 (52.5 kDa) and 18 (45 kDa).
This antibody stains simple and glandular epithelium.
This antibody can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesThis antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
No further titration is required.
We suggest an incubation period of 60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections.
RelevanceFunction: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Similarity: Belongs to the intermediate filament family.
PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.