Mouse monoclonal [5D3] to Cytokeratin 8+18, prediluted
SpecificityThis antibody is specific to human cytokeratins 8 (52.5 kDa) and 18 (45 kDa).
This antibody stains simple and glandular epithelium.
This antibody can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesThis antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
No further titration is required.
We suggest an incubation period of 60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections.
RelevanceCytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with Cytokeratin 18 (type A; acidic subfamily of low molecular weight keratins).