Other Immunogen Type corresponding to Human Cytokeratin 8+18. (Purified keratins).
ab190366 recognizes all simple epithelia and glandular (thyroid, breast, GI tract, respiratory tract, urinary tract). Most squamous carcinomas and all adenocarcinomas are stained by this antibody and keratinizing squamous carcinomas are mostly negative.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/1000.
ab106163 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
1/10 - 1/20.
Incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/50 - 1/100.
Function: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Similarity: Belongs to the intermediate filament family.
PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.