Recombinant
RabMAb

Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966)

Overview

  • Product name
    Anti-Cytokeratin 9 antibody [EPR10932-62]
    See all Cytokeratin 9 primary antibodies
  • Description
    Rabbit monoclonal [EPR10932-62] to Cytokeratin 9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Cytokeratin 9. The exact sequence is proprietary.
    Database link: P35527

  • Positive control
    • NCI-H460, HL60, A431 and HeLa cell lysates; Human skin tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA, PBS
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10932-62
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 62 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly.
    • Tissue specificity
      Expressed in the terminally differentiated epidermis of palms and soles.
    • Involvement in disease
      Defects in KRT9 are a cause of palmoplantar keratoderma epidermolytic (EPPK) [MIM:144200]; also abbreviated as EHPPK. EPPK is a dermatological disorder characterized by diffuse thickening of the epidermis on the entire surface of palms and soles sharply bordered with erythematous margins. Some patients may present with knuckle pads, thick pads of skin appearing over the proximal phalangeal joints.
    • Sequence similarities
      Belongs to the intermediate filament family.
    • Information by UniProt
    • Database links
    • Alternative names
      • CK 9 antibody
      • CK-9 antibody
      • Cytokeratin 9 antibody
      • Cytokeratin-9 antibody
      • Cytokeratin9 antibody
      • EPPK antibody
      • K1C9_HUMAN antibody
      • K9 antibody
      • Keratin 9 antibody
      • Keratin antibody
      • Keratin type I cytoskeletal 9 antibody
      • Keratin-9 antibody
      • Keratin9 antibody
      • KRT 9 antibody
      • Krt9 antibody
      • Spermatid Perinuclear Ring Manchette Protein K9 antibody
      • type I cytoskeletal 9 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human skin tissue, labeling Cytokeratin 9 using ab171966 at a 1/100 dilution.

    • ab171966 showing +ve staining in Human skin tissue.

    • ab171966 showing -ve staining in Human spleen tissue.
    • ab171966 showing -ve staining in Human normal uterus tissue.
    • ab171966 showing -ve staining in Human thyroid gland carcinoma tissue.
    • ab171966 showing -ve staining in Human colon adenocarcinoma tissue.
    • All lanes : Anti-Cytokeratin 9 antibody [EPR10932-62] (ab171966) at 1/1000 dilution

      Lane 1 : NCI-H460 cell lysate
      Lane 2 : HL60 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 62 kDa

    • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling Cytokeratin 9, using ab171966 at 1/10 dilution.

    References

    ab171966 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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