Anti-Cytokeratin antibody [CK-cocktail] (ab115959)

Overview

  • Product nameAnti-Cytokeratin antibody [CK-cocktail]
    See all Cytokeratin primary antibodies
  • Description
    Mouse monoclonal [CK-cocktail] to Cytokeratin
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human epidermal Cytokeratin.

  • Positive control
    • Human skin, lung, colon, prostate, or any GI tissue or cancer tissues.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...
  • ClonalityMonoclonal
  • Clone numberCK-cocktail
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/100.
ICC Use at an assay dependent concentration.

Target

  • FunctionMay regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1).
  • Tissue specificityThe source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.
  • Involvement in diseaseDefects in KRT1 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
    Defects in KRT1 are the cause of ichthyosis hystrix Curth-Macklin type (IHCM) [MIM:146590]. IHCM is a genodermatosis with severe verrucous hyperkeratosis. Affected individuals manifest congenital verrucous black scale on the scalp, neck, and limbs with truncal erythema, palmoplantar keratoderma and keratoses on the lips, ears, nipples and buttocks.
    Defects in KRT1 are a cause of palmoplantar keratoderma non-epidermolytic (NEPPK) [MIM:600962]. NEPKK is a dermatological disorder characterized by focal palmoplantar keratoderma with oral, genital, and follicular lesions.
    Defects in KRT1 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
    Defects in KRT1 are the cause of palmoplantar keratoderma striate type 3 (SPPK3) [MIM:607654]; also known as keratosis palmoplantaris striata III. SPPK3 is a dermatological disorder affecting palm and sole skin where stratum corneum and epidermal layers are thickened. There is no involvement of non-palmoplantar skin, and both hair and nails are normal.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    Undergoes deimination of some arginine residues (citrullination).
  • Cellular localizationCell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • 67 kDa cytokeratin antibody
    • CK-1 antibody
    • CK1 antibody
    • Cytokeratin 1 antibody
    • Cytokeratin 19 antibody
    • Cytokeratin 8 antibody
    • Cytokeratin-1 antibody
    • EHK antibody
    • EHK1 antibody
    • EPPK antibody
    • Hair alpha protein antibody
    • K1 antibody
    • K2C1_HUMAN antibody
    • Keratin 1 antibody
    • Keratin 19 antibody
    • Keratin 8 antibody
    • Keratin antibody
    • Keratin-1 antibody
    • Krt1 antibody
    • KRT19 antibody
    • KRT1A antibody
    • KRT8 antibody
    • KRTA antibody
    • NEPPK antibody
    • type II cytoskeletal 1 antibody
    • Type-II keratin Kb1 antibody
    see all

References for Anti-Cytokeratin antibody [CK-cocktail] (ab115959)

This product has been referenced in:
  • Combes AN  et al. Cap mesenchyme cell swarming during kidney development is influenced by attraction, repulsion, and adhesion to the ureteric tip. Dev Biol 418:297-306 (2016). ICC/IF . Read more (PubMed: 27346698) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry. I have confirmed with the laboratory that this Cytokeratin cocktail contains 5 different clones. The immunogen used to generate these was Human epidermal keratin. The expected MW of cytokeratins are: CK1 (67), CK2 (65.5...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"