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ab169811 is for the simultaneous detection of 36 Human Cytokines. Suitable for all liquid sample types.
Targets: BDNF, BLC, Ck beta 8-1, Eotaxin-1, Eotaxin-2, Eotaxin-3, FGF-4, FGF-6, FGF-7, FGF-9, Flt-3 Ligand, Fractalkine, GCP-2, GDNF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL16, IP-10, LIF, Light, MCP-4, MIF, MIP-3 alpha, NAP-2, NT-3, NT-4, Osteoprotegerin, PARC, PLGF, TGF beta 2, TGF beta 3, TIMP-1, TIMP-2
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
|Components||1 x 4 Membranes||1 x 8 Membranes|
|1,000X HRP-Streptavidin Buffer||1 x 50µl||1 x 50µl|
|1X Blocking Buffer||1 x 25ml||1 x 50ml|
|20X Wash Buffer I||1 x 10ml||1 x 20ml|
|20X Wash Buffer II||1 x 10ml||1 x 20ml|
|2X Cell Lysis Buffer||1 x 10ml||1 x 20ml|
|8-Well Incubation Tray (with Lid)||1 unit||1 unit|
|Biotinylated Antibody Cocktail||2 vials||4 vials|
|Cytokine Antibody Array Membranes||4 units||8 units|
|Detection Buffer C||1 x 1.5ml||1 x 2.5ml|
|Detection Buffer D||1 x 1.5ml||1 x 2.5ml|
Our Abpromise guarantee covers the use of ab169811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Multiplex Protein Detection||Use at an assay dependent concentration.|
ab169811 has not yet been referenced specifically in any publications.