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ab169817 is for the simultaneous detection of 60 Human Cytokines. Suitable for all sample types.
Targets: Angiogenin, BDNF, BLC, BMP-4, BMP-6, CK beta 8-1, CNTF, EGF, Eotaxin-1, Eotaxin-2, Eotaxin-3, FGF-6, FGF-7, Flt-3 Ligand, Fractalkine, GCP-2, GDNF, GM-CSF, I-309, IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-4, IGF-1, IL-10, IL-13, IL-15, IL-16, IL-1 alpha, IL-1 beta, IL-1 ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, Leptin, Light, MCP-1, MCP-2, MCP-3, MCP-4, M-CSF, MDC, MIG, MIP-1 delta, MIP-3 alpha, NAP-2, NT-3, PARC, PDGF-BB, RANTES, SCF, SDF-1, TARC, TGF beta 1, TGF beta 3, TNF alpha, TNF beta
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
|Components||1 x 4 Membranes||1 x 8 Membranes||1 x 2 Membranes|
|1,000X HRP-Streptavidin Buffer||1 x 50µl||1 x 50µl||1 x 50µl|
|1X Blocking Buffer||1 x 25ml||1 x 50ml||1 x 25ml|
|20X Wash Buffer I||1 x 10ml||1 x 20ml||1 x 10ml|
|20X Wash Buffer II||1 x 10ml||1 x 20ml||1 x 10ml|
|2X Cell Lysis Buffer||1 x 10ml||1 x 20µg||1 x 10ml|
|8-Well Incubation Tray (with Lid)||1 unit||1 unit||1 unit|
|Biotinylated Antibody Cocktail (C6)||2 vials||4 vials||2 vials|
|Human Cytokine Antibody Array Membranes (C6)||4 units||8 units||2 units|
|Detection Buffer C||1 x 1.5ml||1 x 2.5ml||1 x 1.5ml|
|Detection Buffer D||1 x 1.5ml||1 x 2.5ml||1 x 1.5ml|
Our Abpromise guarantee covers the use of ab169817 in the following tested applications.
|Multiplex Protein Detection||Use at an assay dependent concentration.|
Left image: Conditioned media from MCF-7 cells cultured in 2D; right image: Conditioned media from MCF-7 cells cultured in 3D”
The array was used to examine the potential differences in cytokine profile expression in 2D and 3D culture breast cancer cells (MCF-7), which may account for the significant upregulation of hepcidin (an iron export regulatory protein) observed in 3D culture. Experiments were performed according to the manufacturer’s instructions for the array, using cell lysate and conditioned media. Samples and biotin-conjugated anti-cytokines were incubated overnight at 4°C. Since many cytokines were detected at low levels when imaged, a comparison between 2D and 3D culture was not conclusive. Conditioned media and cell lysates yielded similar results.”
Rating : 4/5. Product is recommended to anyone seeking information on production of a broad range of cytokines. Product is very easy to use.
Verified user: Nicole Blanchette, Graduate Assistant, Molecular Biology & Biophysics, UConnHealth, Farmington, CT, USA.
Left image: Conditioned media from iPSC-derived astrocytes; right image: Media only control.
Samples were incubated overnight at 4C as recommended and included the large volume wash. Images were captured using CCD camera for the exposure times indicated on the image.
Rating 5/5. Simple, sensitive and accurate method to detect multiple cytokines and growth factors from a single sample. The membranes were also consistent across the batch which allowed me to test several samples in parallel. Highly recommended.
ab169817 has not yet been referenced specifically in any publications.