Cytokine Array – Human Cytokine Antibody Array (Membrane, 60 targets) (ab169817)

Overview

  • Product name
    Cytokine Array – Human Cytokine Antibody Array (Membrane, 60 targets)
    See all Cytokine Antibody Array antibody arrays
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Semi-quantitative
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab169817 is for the simultaneous detection of 60 Human Cytokines. Suitable for all sample types.


    Targets: Angiogenin, BDNF, BLC, BMP-4, BMP-6, CK beta 8-1, CNTF, EGF, Eotaxin-1, Eotaxin-2, Eotaxin-3, FGF-6, FGF-7, Flt-3 Ligand, Fractalkine, GCP-2, GDNF, GM-CSF, I-309, IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-4, IGF-1, IL-10, IL-13, IL-15, IL-16, IL-1 alpha, IL-1 beta, IL-1 ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, Leptin, Light, MCP-1, MCP-2, MCP-3, MCP-4, M-CSF, MDC, MIG, MIP-1 delta, MIP-3 alpha, NAP-2, NT-3, PARC, PDGF-BB, RANTES, SCF, SDF-1, TARC, TGF beta 1, TGF beta 3, TNF alpha, TNF beta


    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


    Learn more about membrane antibody arrays

  • Notes

    If you are interested in this cytokine array, arrays ab133997 and ab133998 may also be of interest.


    A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

  • Tested applications
    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes
    1,000X HRP-Streptavidin Buffer 1 x 50µl 1 x 50µl 1 x 50µl
    1X Blocking Buffer 1 x 25ml 1 x 50ml 1 x 25ml
    20X Wash Buffer I 1 x 10ml 1 x 20ml 1 x 10ml
    20X Wash Buffer II 1 x 10ml 1 x 20ml 1 x 10ml
    2X Cell Lysis Buffer 1 x 10ml 1 x 20µg 1 x 10ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit
    Biotinylated Antibody Cocktail (C6) 2 vials 4 vials 2 vials
    Human Cytokine Antibody Array Membranes (C6) 4 units 8 units 2 units
    Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
    Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Left image: Conditioned media from MCF-7 cells cultured in 2D; right image: Conditioned media from MCF-7 cells cultured in 3D”

    The array was used to examine the potential differences in cytokine profile expression in 2D and 3D culture breast cancer cells (MCF-7), which may account for the significant upregulation of hepcidin (an iron export regulatory protein) observed in 3D culture. Experiments were performed according to the manufacturer’s instructions for the array, using cell lysate and conditioned media. Samples and biotin-conjugated anti-cytokines were incubated overnight at 4°C. Since many cytokines were detected at low levels when imaged, a comparison between 2D and 3D culture was not conclusive. Conditioned media and cell lysates yielded similar results.”

    Rating : 4/5. Product is recommended to anyone seeking information on production of a broad range of cytokines. Product is very easy to use.

    Verified user: Nicole Blanchette, Graduate Assistant, Molecular Biology & Biophysics, UConnHealth, Farmington, CT, USA.

  • Left image: Conditioned media from iPSC-derived astrocytes; right image: Media only control.

    Samples were incubated overnight at 4C as recommended and included the large volume wash. Images were captured using CCD camera for the exposure times indicated on the image.

    Rating 5/5. Simple, sensitive and accurate method to detect multiple cytokines and growth factors from a single sample. The membranes were also consistent across the batch which allowed me to test several samples in parallel. Highly recommended.

Protocols

References

ab169817 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Questions for ab169817.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up