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ab193659 can be used for the simultaneous detection of 96 Mouse Cytokine proteins in serum, plasma, cell culture media and other liquid samples types. It comes as 1 kit including 2 separate membranes labelled C3 and C4 (see below for images showing the array layout on each membrane).
Target names: Axl, Bfgf, BLC (CXCL13), CD30 Ligand (TNFSF8), CD30 (TNFRSF8), CD40 (TNFRSF5), CRG-2, CTACK (CCL27), CXCL16, CD26 (DPPIV), Dtk, Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), E-Selectin, Fas Ligand (TNFSF6), Fc gamma RIIB (CD32b), Flt-3 Ligand, Fractalkine (CX3CL1), GCSF, GITR (TNFRSF18), GM-CSF, HGFR, ICAM-1 (CD54) IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IGF-1, IGF-2, IL-1 beta (IL-1 F2), IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-15, IL-17A, IL-17 RB, IL-1 alpha (IL-1 F1), IL-2, IL-3, IL-3 R beta, IL-4, IL-5, IL-6, IL-7, IL-9, I-TAC (CXCL11), KC (CXCL1), Leptin, Leptin R, LIX, L-Selectin (CD62L), Lungkine (CXCL15), Lymphotactin (XCL1), MCP-1 (CCL2), MCP-5, M-CSF, MDC (CCL22), MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 gamma, MIP-2, MIP-3 beta (CCL19), MIP-3 alpha (CCL20), MMP-2, MMP-3, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), Platelet Factor 4 (CXCL4), Pro-MMP-9, P-Selectin, RANTES (CCL5), Resistin, SCF, SDF-1 alpha (CXCL12 alpha), Sonic Hedgehog N-Terminal (Shh-N), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TARC (CCL17), I-309 (TCA-3/CCL1), TECK (CCL25), TCK-1 (CXCL7), TIMP-1, TIMP-2, TNF alpha, Thrombopoietin (TPO), TRANCE (TNFSF11), TROY (TNFRSF19), TSLP, VCAM-1 (CD106), VEGF-A, VEGFR1, VEGFR2, VEGFR3, VEGF-D
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
A table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
|Components||1 x 4 Membranes||1 x 8 Membranes||1 x 2 Membranes|
|1,000X HRP-Streptavidin Buffer||1 x 50µl||2 x 50µl||1 x 50µl|
|1X Blocking Buffer||2 x 25ml||4 x 25ml||1 x 25ml|
|20X Wash Buffer I||1 x 20ml||2 x 20ml||1 x 10ml|
|20X Wash Buffer II||1 x 20ml||2 x 20ml||1 x 10ml|
|2X Cell Lysis Buffer||1 x 16ml||2 x 16ml||1 x 10ml|
|8-Well Incubation Tray (with Lid)||1 unit||2 units||1 unit|
|Biotinylated Antibody Cocktail (C3)||2 vials||4 vials||1 vial|
|Biotinylated Antibody Cocktail (C4)||2 vials||4 vials||1 vial|
|Detection Buffer C||1 x 2.5ml||2 x 2.5ml||1 x 1.5ml|
|Detection Buffer D||1 x 2.5ml||2 x 2.5ml||1 x 1.5ml|
|Mouse Cytokine Antibody Arrays Membranes (C3)||1 x 4 units||1 x 8 units||1 x 2 units|
|Mouse Cytokine Antibody Arrays Membranes (C4)||1 x 4 units||1 x 8 units||1 x 2 units|
Our Abpromise guarantee covers the use of ab193659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Multiplex Protein Detection||Use at an assay dependent concentration.|
ab193659 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"