Cytokine Array – Mouse Cytokine Antibody Array (Membrane, 96 Targets) (ab193659)

Overview

  • Product name
    Cytokine Array – Mouse Cytokine Antibody Array (Membrane, 96 Targets)
    See all Cytokine Antibody Array antibody arrays
  • Sample type
    Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
  • Assay type
    Semi-quantitative
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab193659 can be used for the simultaneous detection of 96 Mouse Cytokine proteins in serum, plasma, cell culture media and other liquid samples types. It comes as 1 kit including 2 separate membranes labelled C3 and C4 (see below for images showing the array layout on each membrane). 


    Target names: Axl, Bfgf, BLC (CXCL13), CD30 Ligand (TNFSF8), CD30 (TNFRSF8), CD40 (TNFRSF5), CRG-2, CTACK (CCL27), CXCL16, CD26 (DPPIV), Dtk, Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), E-Selectin, Fas Ligand (TNFSF6), Fc gamma RIIB (CD32b), Flt-3 Ligand, Fractalkine (CX3CL1), GCSF, GITR (TNFRSF18), GM-CSF, HGFR, ICAM-1 (CD54) IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IGF-1, IGF-2, IL-1 beta (IL-1 F2), IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-15, IL-17A, IL-17 RB, IL-1 alpha (IL-1 F1), IL-2, IL-3, IL-3 R beta, IL-4, IL-5, IL-6, IL-7, IL-9, I-TAC (CXCL11), KC (CXCL1), Leptin, Leptin R, LIX, L-Selectin (CD62L), Lungkine (CXCL15), Lymphotactin (XCL1), MCP-1 (CCL2), MCP-5, M-CSF, MDC (CCL22), MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 gamma, MIP-2, MIP-3 beta (CCL19), MIP-3 alpha (CCL20), MMP-2, MMP-3, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), Platelet Factor 4 (CXCL4), Pro-MMP-9, P-Selectin, RANTES (CCL5), Resistin, SCF, SDF-1 alpha (CXCL12 alpha), Sonic Hedgehog N-Terminal (Shh-N), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TARC (CCL17), I-309 (TCA-3/CCL1), TECK (CCL25), TCK-1 (CXCL7), TIMP-1, TIMP-2, TNF alpha, Thrombopoietin (TPO), TRANCE (TNFSF11), TROY (TNFRSF19), TSLP, VCAM-1 (CD106), VEGF-A, VEGFR1, VEGFR2, VEGFR3, VEGF-D


    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


    Learn more about membrane antibody arrays

  • Notes

    A table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

  • Tested applications
    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes
    1,000X HRP-Streptavidin Buffer 1 x 50µl 2 x 50µl 1 x 50µl
    1X Blocking Buffer 2 x 25ml 4 x 25ml 1 x 25ml
    20X Wash Buffer I 1 x 20ml 2 x 20ml 1 x 10ml
    20X Wash Buffer II 1 x 20ml 2 x 20ml 1 x 10ml
    2X Cell Lysis Buffer 1 x 16ml 2 x 16ml 1 x 10ml
    8-Well Incubation Tray (with Lid) 1 unit 2 units 1 unit
    Biotinylated Antibody Cocktail (C3) 2 vials 4 vials 1 vial
    Biotinylated Antibody Cocktail (C4) 2 vials 4 vials 1 vial
    Detection Buffer C 1 x 2.5ml 2 x 2.5ml 1 x 1.5ml
    Detection Buffer D 1 x 2.5ml 2 x 2.5ml 1 x 1.5ml
    Mouse Cytokine Antibody Arrays Membranes (C3) 1 x 4 units 1 x 8 units 1 x 2 units
    Mouse Cytokine Antibody Arrays Membranes (C4) 1 x 4 units 1 x 8 units 1 x 2 units
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab193659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Typical images obtained with  Abcam Mouse Cytokine Antibody Array - Membrane (96 Targets) ab193659. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

Protocols

References

ab193659 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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