Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)

Overview

  • Product nameAnti-Cytomegalovirus Glycoprotein B antibody [2F12]
    See all Cytomegalovirus Glycoprotein B primary antibodies
  • Description
    Mouse monoclonal [2F12] to Cytomegalovirus Glycoprotein B
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Immunogen

    Cytomegalovirus gB Envelpe Protein (UL55, gp58/116, gC-1 and glycoprotein B)

  • EpitopeEpitope has not been mapped.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPhosphate buffered saline
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number2F12
  • MyelomaNS1/1-Ag4-1
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC/IF
  • Application notesImmunofluoresence titer 1+ @ 1:12,800, IFA is tested with acetone fixtation.
    ELISA titer 0.100 @ 1:102,400
    Western Blot > 1:4,000
  • Target

    • RelevanceHuman cytomegalovirus (HCMV) glycoprotein B (gB) is an abundant virion envelope protein that has been shown to be essential for the infectivity of HCMV. HCMV gB is also one of the most immunogenic virus-encoded proteins, and a significant fraction of virus neutralizing antibodies are directed at gB.
    • Cellular localizationVirus envelope.
    • Alternative names
      • CMV glycoprotein B antibody
      • Glycoprotein B antibody
      • HCMV antibody
      • HHV5 glycoprotein B antibody
      • UL55 antibody
      see all

    Anti-Cytomegalovirus Glycoprotein B antibody [2F12] images

    • See protocol for procedure. Procedure uses 10-well CMV slides and anti-Mouse-FITC F(ab')2 w/ counterstain.

    • See protocol for procedure. Procedure uses 10-well CMV slides and anti-Mouse-FITC F(ab')2 w/ counterstain.

    • Determined by IFA (see protocol section) at an antibody dilution of 1:100 with anti-Mouse-FITC F(ab')2 w/ counterstain.

    • See protocol for procedure. Antigen is CMV Infected Cell Extract coated at a dilution of 1:100.

    • See protocol for procedure. Antigen is CMV Infected Cell Extract at 10 µg/cm.

    References for Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)

    This product has been referenced in:
    • Murrell I  et al. Impact of sequence variation in the UL128 locus on production of human cytomegalovirus in fibroblast and epithelial cells. J Virol 87:10489-500 (2013). WB . Read more (PubMed: 23885075) »
    • Haspot F  et al. Human cytomegalovirus entry into dendritic cells occurs via a macropinocytosis-like pathway in a pH-independent and cholesterol-dependent manner. PLoS One 7:e34795 (2012). WB . Read more (PubMed: 22496863) »

    See all 3 Publications for this product

    Product Wall

    Antibody ab54023 has been tested in a neutralization assay that is described in the following reference. Figure 4 shows the data for this clone.

    Kirchmeier M et al. Enveloped virus-like particle expression of human cytomegalovirus glycoprotein...

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    Unfortunately, the Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499), this antibody has not as yet been used in staining paraffin embedded sections to our knowledge. We do not have any other antibodies a...

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    Thank you for contacting us.

    The cells are human fibroblast infected with CMV (AD169) - acetone fixed after 48 hours of infection

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (AD169-infected HFFF)
    Loading amount 100000 cells
    Specification AD169-infected HFFF
    Treatment mock/endoH/PNGaseF for 16H
    Gel Running Conditions Reduced Denaturing
    Blocking step Superblock Buffer (Pierce) as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
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    Abcam user community

    Verified customer

    Submitted Feb 12 2008

    This antibody has never been tested against Rhesus CMV and 64% homology does not sound very promising - you would be better off with a polyclonal antibody. We are in the final stages of characterizing a chicken anti-CMV that may be applicable - will...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"