Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)


  • Product nameAnti-Cytomegalovirus Glycoprotein B antibody [2F12]
    See all Cytomegalovirus Glycoprotein B primary antibodies
  • Description
    Mouse monoclonal [2F12] to Cytomegalovirus Glycoprotein B
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Immunogen

    Cytomegalovirus gB Envelpe Protein (UL55, gp58/116, gC-1 and glycoprotein B)

  • EpitopeEpitope has not been mapped.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number2F12
  • MyelomaNS1/1-Ag4-1
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab6499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesImmunofluoresence titer 1+ @ 1:12,800, IFA is tested with acetone fixtation.
    ELISA titer 0.100 @ 1:102,400
    Western Blot > 1:4,000
  • Target

    • RelevanceHuman cytomegalovirus (HCMV) glycoprotein B (gB) is an abundant virion envelope protein that has been shown to be essential for the infectivity of HCMV. HCMV gB is also one of the most immunogenic virus-encoded proteins, and a significant fraction of virus neutralizing antibodies are directed at gB.
    • Cellular localizationVirus envelope.
    • Alternative names
      • CMV glycoprotein B antibody
      • Glycoprotein B antibody
      • HCMV antibody
      • HHV5 glycoprotein B antibody
      • UL55 antibody
      see all

    Anti-Cytomegalovirus Glycoprotein B antibody [2F12] images

    • See protocol for procedure. Procedure uses 10-well CMV slides and anti-Mouse-FITC F(ab')2 w/ counterstain.

    • See protocol for procedure. Procedure uses 10-well CMV slides and anti-Mouse-FITC F(ab')2 w/ counterstain.

    • Determined by IFA (see protocol section) at an antibody dilution of 1:100 with anti-Mouse-FITC F(ab')2 w/ counterstain.

    • See protocol for procedure. Antigen is CMV Infected Cell Extract coated at a dilution of 1:100.

    • See protocol for procedure. Antigen is CMV Infected Cell Extract at 10 µg/cm.

    References for Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499)

    This product has been referenced in:
    • Murrell I  et al. Impact of sequence variation in the UL128 locus on production of human cytomegalovirus in fibroblast and epithelial cells. J Virol 87:10489-500 (2013). WB . Read more (PubMed: 23885075) »
    • Haspot F  et al. Human cytomegalovirus entry into dendritic cells occurs via a macropinocytosis-like pathway in a pH-independent and cholesterol-dependent manner. PLoS One 7:e34795 (2012). WB . Read more (PubMed: 22496863) »

    See all 3 Publications for this product

    Product Wall

    Antibody ab54023 has been tested in a neutralization assay that is described in the following reference. Figure 4 shows the data for this clone.

    Kirchmeier M et al. Enveloped virus-like particle expression of human cytomegalovirus glycoprotein...

    Read More

    Thank you for contacting us.

    Unfortunately, the Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499), this antibody has not as yet been used in staining paraffin embedded sections to our knowledge. We do not have any other antibodies a...

    Read More

    Thank you for contacting us.

    The cells are human fibroblast infected with CMV (AD169) - acetone fixed after 48 hours of infection

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (AD169-infected HFFF)
    Loading amount 100000 cells
    Specification AD169-infected HFFF
    Treatment mock/endoH/PNGaseF for 16H
    Gel Running Conditions Reduced Denaturing
    Blocking step Superblock Buffer (Pierce) as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Feb 12 2008

    This antibody has never been tested against Rhesus CMV and 64% homology does not sound very promising - you would be better off with a polyclonal antibody. We are in the final stages of characterizing a chicken anti-CMV that may be applicable - will...

    Read More