Anti-Cytomegalovirus Glycoprotein B antibody [HCMV37] (ab54023)

Overview

  • Product name
    Anti-Cytomegalovirus Glycoprotein B antibody [HCMV37]
    See all Cytomegalovirus Glycoprotein B primary antibodies
  • Description
    Mouse monoclonal [HCMV37] to Cytomegalovirus Glycoprotein B
  • Specificity
    This antibody is specific for Cytomegalovirus glycoprotein B late neutralizing antigen. Reacts with MRC-5 infected cells but not uninfected cells.
  • Tested applications
    Suitable for: ELISA, ICC/IF, IHC-Fr, Neutralisingmore details
  • Species reactivity
    Reacts with Cytomegalovirus
  • Immunogen

    Human CMV extracts

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    HCMV37
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC/IF
IHC-Fr
Neutralising
  • Application notes
    ELISA: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution (CMV infected cells).
    IHC-Fr: Use at an assay dependent dilution (CMV infected cells).
    Neut: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Human cytomegalovirus (HCMV) glycoprotein B (gB) is an abundant virion envelope protein that has been shown to be essential for the infectivity of HCMV. HCMV gB is also one of the most immunogenic virus-encoded proteins, and a significant fraction of virus neutralizing antibodies are directed at gB.
    • Cellular localization
      Virus envelope.
    • Alternative names
      • CMV glycoprotein B antibody
      • Glycoprotein B antibody
      • HCMV antibody
      • HHV5 glycoprotein B antibody
      • UL55 antibody
      see all

    Images

    • ab54023, staining Cytomegalovirus Glycoprotein B in monocyte-derived dendritic cells infected with Human cytomegalovirus (VHL/E strain), cultured for 3 hours (left panels) and 24 hours (right panels), by Immunocytochemistry/ Immunofluorescence.

      Cells were fixed with paraformaldehyde and permeabilized with 0.2% Triton X-100 in PBS for 10 minutes at room temperature. Samples were incubated with primary antibody and an AlexaFluor®568-conjugated goat anti-mouse monoclonal IgG was used as the secondary antibody.

    References

    This product has been referenced in:
    • Kirchmeier M  et al. Enveloped virus-like particle expression of human cytomegalovirus glycoprotein B antigen induces antibodies with potent and broad neutralizing activity. Clin Vaccine Immunol 21:174-80 (2014). Neutralising . Read more (PubMed: 24334684) »
    • Haspot F  et al. Human cytomegalovirus entry into dendritic cells occurs via a macropinocytosis-like pathway in a pH-independent and cholesterol-dependent manner. PLoS One 7:e34795 (2012). ICC/IF . Read more (PubMed: 22496863) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Antibody ab54023 has been tested in a neutralization assay that is described in the following reference. Figure 4 shows the data for this clone.

    Kirchmeier M et al. Enveloped virus-like particle expression of human cytomegalovirus glycoprotein...

    Read More

    This antibody was derived from ascites fluid.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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