Anti-Cytomegalovirus IE1 72 antibody [IE1.G10] (ab30924)

Overview

  • Product nameAnti-Cytomegalovirus IE1 72 antibody [IE1.G10]
  • Description
    Mouse monoclonal [IE1.G10] to Cytomegalovirus IE1 72
  • SpecificityRecognize specifically the major immediate-early (IE) gene products of HCMV IE1-72.
  • Tested applicationsSuitable for: WB, ELISA, IPmore details
  • Immunogen

    Recombinant IE1 protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberIE1.G10
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab30924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 10 kDa.
ELISA 1/2000 - 1/5000.
IP Use at an assay dependent dilution.

Target

  • RelevanceHuman cytomegalovirus (HCMV) is a member of the beta class of human herpesviruses and is associated with a number of serious medical conditions, particularly in individuals with compromised immune systems. Its genome is the largest of the human herpesviruses (240 kb) and has the capacity to encode over 200 distinct viral gene products. Results from a number of different laboratories have indicated that two of these gene products, the major immediate-early (IE) gene products of HCMV (IE1-72 and IE2-86), are essential viral proteins that regulate expression of a variety of viral and heterologous genes at the level of transcription . IE1-72 and IE2-86 have been demonstrated to act synergistically to mediate transcriptional activation of several viral genes of subsequent kinetic classes, including viral early genes, such as the viral DNA polymerase gene (and other early genes), as well as early-late genes, such as pp65. Both IE1-72 and IE2-86 have the capacity to regulate transcription from viral and cellular promoters through interactions with cellular activator proteins, such as E2F, p53, CAAT-box-binding factor, CREB-binding factor, and probably others.
  • Cellular localizationNuclear. Host nucleus. Colocalizes with host PML-associated nuclear bodies
  • Alternative names
    • 55 kDa immediate-early protein 1 antibody
    • HCMV antibody
    • HHV 5 antibody
    • Human herpesvirus 5 antibody
    • IE1 antibody
    • Immediate early protein1 antibody
    • Regulatory protein IE1 antibody
    • UL123 antibody
    • VIE1_HCMVA antibody
    see all

References for Anti-Cytomegalovirus IE1 72 antibody [IE1.G10] (ab30924)

This product has been referenced in:
  • Mücke K  et al. Human cytomegalovirus major immediate early 1 protein targets host chromosomes by docking to the acidic pocket on the nucleosome surface. J Virol 88:1228-48 (2014). ICC/IF, WB ; Human . Read more (PubMed: 24227840) »

See 1 Publication for this product

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DISCOUNT CODE: xxxxxx
Expiration date: 27. August 2012


I am very pleased to hear you would like to accept our offer and test ab30924 in CHIP. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this...

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For ab30924 Anti-Cytomegalovirus IE1 72 antibody [IE1.G10], the immunogen is a full recombinant HCMV (Human Cytomagalo Virus) IE pp72 antigen. The exact epitope is unknown as a full protein was use...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"