Anti-Cytomegalovirus pp65 antibody [B2] (ab8250)


  • Product nameAnti-Cytomegalovirus pp65 antibody [B2]
    See all Cytomegalovirus pp65 primary antibodies
  • Description
    Mouse monoclonal [B2] to Cytomegalovirus pp65
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Immunogen

    This clone has been derived from hybridization of P3-X63.653 myeloma cells with spleen cells of a Balb/c mice immunised with early proteins of cytomegalovirus

  • EpitopeThis antibody reacts with 65 kDa protein (pp65) of cytomegalovirus


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberB2
  • MyelomaP3x63-Ag8.653
  • IsotypeIgG2a
  • Light chain typeunknown
  • Research areas


Our Abpromise guarantee covers the use of ab8250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA
    Western blotting.
  • Target

    • RelevanceCytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period. CMV viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. pp65 is a 65kD phosphorylated glycoprotein and is the most abundant of the late antigens.
    • Alternative names
      • 65 kDa matrix phosphoprotein antibody
      • 65 kDa phosphoprotein antibody
      • Tegument protein pp65 antibody
      • Tegument protein UL83 antibody

    References for Anti-Cytomegalovirus pp65 antibody [B2] (ab8250)

    ab8250 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your inquiry. As you may have seen already, we have a number of HCMV pp65 antibodies available in our catalog (such as ab6503, ab8250, ab31624, ab49214, ab53489). These were raised against the pp65 protein, however, unfortunately the ...

    Read More

    We don't have any information about the antibody being used for that type of ELISA experiment so I unfortunately cannot make any recommendations.

    Thank you for your enquiry. You did not mention what application you would be using ab8250 in but for Western blotting I would recommend starting at 1 ug/ml and optimizing from there. If you have any more questions please contact us again.