Anti-Cytomegalovirus pp65 antibody [CH12] (ab53489)

Overview

  • Product nameAnti-Cytomegalovirus pp65 antibody [CH12]
    See all Cytomegalovirus pp65 primary antibodies
  • Description
    Mouse monoclonal [CH12] to Cytomegalovirus pp65
  • Specificityab53489 is reactive with pp65 (UL83) of Cytomegalovirus.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with Cytomegalovirus.
  • Immunogen

    HCMV AD169 IC Extract.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody originates from ascites fluids and is purified by protein G agarose affinity chromatography.
  • ClonalityMonoclonal
  • Clone numberCH12
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 10 µg/ml.

Target

  • RelevanceCytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period. CMV viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. pp65 is a 65kD phosphorylated glycoprotein and is the most abundant of the late antigens.
  • Alternative names
    • 65 kDa matrix phosphoprotein antibody
    • 65 kDa phosphoprotein antibody
    • Tegument protein pp65 antibody
    • Tegument protein UL83 antibody

References for Anti-Cytomegalovirus pp65 antibody [CH12] (ab53489)

This product has been referenced in:
  • Walker JD  et al. Cytomegalovirus-infected human endothelial cells can stimulate allogeneic CD4+ memory T cells by releasing antigenic exosomes. J Immunol 182:1548-59 (2009). WB . Read more (PubMed: 19155503) »

See 1 Publication for this product

Product Wall

Thank you for your reply.

The polyclonal isotope control will work with the monoclonal antibody. You should have no issues using this combination.

Please let me know if there is anything else I can help you with.

Thank you for your reply.

The secondary antibody ab130782 would be a good choice to use with ab53495, and the isotope that I mentioned previously would also work well.

If I can help you select any other reagents, or if there is anyt...

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Thank you for contacting Abcam.

For ab53489, I would recommend the following mouse IgG Isotype control:

http://www.abcam.com/Mouse-IgG-ab37355.html



If there is anything else I can help you with, please let me know.

Thank you for your inquiry. As you may have seen already, we have a number of HCMV pp65 antibodies available in our catalog (such as ab6503, ab8250, ab31624, ab49214, ab53489). These were raised against the pp65 protein, however, unfortunately the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"