CytoPainter F-actin Staining Kit - Red Fluorescence (ab112127)

Overview

  • Product nameCytoPainter F-actin Staining Kit - Red Fluorescence
    See all F-actin kits
  • Tests
    1 x 500 test
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay typeCell-based
  • Species reactivity
    Reacts with: Human
    Predicted to work with: all Mammals
  • Product overview

    Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.

    ab112127 is designed to stain F-actins in fixed cells with red fluorescence. The red fluorescent phalloidin conjugate, which is selectively bound to F-actins, is a high-affinity probe for F-actins. The red fluorescent phalloidin conjugate has Ex/Em = 594/610 nm. Used at nanomolar concentrations, phallotoxins can be conveniently used to label, identify and quantitate F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. The red fluorescent phalloidin conjugate has good thermal and photo stability.

    ab112127 provides all the essential components with an optimized labeling protocol. It is an excellent tool for preserving fluorescent images of particular cells, and can also be used for fluorescence microscope demonstrations.

  • Notes

    ab112127 should be stored dessicated.

  • Tested applicationsSuitable for: FM, ICC/IFmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab112127 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    FM Use at an assay dependent concentration.
    ICC/IF Use at an assay dependent concentration.

    CytoPainter F-actin Staining Kit - Red Fluorescence images

    •  F-actin staining (red) in chondrocytes in growth plate cartilage. Mouse femur bone was fixed with 4% PFA for 72 hours, decalcified and cut into 8-10 µm sections in frozen. Tissue was then stained for 60 minutes with ab112127 before a final wash in PBS.

       

      This image is courtesy of an anonymous Abreview

    • HeLa cells were fixed with 4% PFA for 10 minutes, rinsed with PBS and stained for 60 minutes with ab112127 before a final wash in PBS prior to mounting slides.

      Images obtained with an Olympus BX61 microscope using a Texas Red filter (595/613nm) - 50ms exposure.

    • Images of CPA cells fixed with formaldehyde and stained with ab112127 in a black 96-well plate Left image: Cells labeled with 1X Red Fluorescent Phalloidin Conjugate for 30 min only. Right image: Cells treated with phalloidin for 10 min, then stained with Red Fluorescent Phalloidin Conjugate for 30 min.

    Protocols

    References for CytoPainter F-actin Staining Kit - Red Fluorescence (ab112127)

    ab112127 has not yet been referenced specifically in any publications.

    Product Wall

    Cytopainter F-actin in Hydractinia

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    We used cytopainter F-actin staining kit to study morphology in the model organism Hydractinia during head regeneration. We fixed our whole animals in 4% pararfomaldehde in PBS followed by three ten minute washes in 0.1%triton in PBS. The animals were incubated in component in at a 1:500 dilution in component B for 45 minutes. They were then washed in PBS three times and mounted in fluorescent mounting medium.
    The picture shows F-actin staining(Red) and Hoescht nuclear staining(blue). The image was taken on a spinning disc confocal microscope.
    Username

    Mr. James Gahan

    Verified customer

    Submitted Jan 23 2014

    Yes, I believe they are also in the bone matrix.

    http://www.ncbi.nlm.nih.gov/pubmed/9610818.

    Yes, it does. Please see attached reference.





    http://www.ncbi.nlm.nih.gov/pubmed/9610818



    http://cjasn.asnjournals.org/content/3/Supplement_3/S131.full.pdf+html

    Thank you for contacting us.

    The CytoPainter kits are compatible with secondary antibodies and nuclear dyes for multicolor staining or GFP-transfected cells, for example. The lab has confirmed that these kits can be used with other antibodies...

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    Thank you for contacting us.

    This kit has an excitation of 583 nm and an emission at 603 nm. I have contacted the lab who confirm that this kit can be used with other antibodies providing that its emission does not overlap with the kits emiss...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    Thank you for your reply and I am sorry about the confusion over the component list and the protocol.

    As you have added the Methanol to the already reconstituted Component A, I would be happy to send you a new kit, as I am sure if the Componen...

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    Thank you for contacting Abcam.

    I am sorry about the mix up, but it seems as though you received the older version of the protocol. Also, in the newer version of the kit, there is only Component A & B, and component A is already in a liqui...

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    Vielen Dank für Ihren Anruf.

    Ich freue mich Ihnen bestätigen zu können, dass die 50x Stock Solution von Red Fluorescent Phalloidin Conjugate für sechs Monate bei -20C aufgehoben werden kann.

    Ich hoffe diese Infor...

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    Thank you for contacting Abcam. The CytoPainter F-actin Staining Kit - Red Fluorescence kit will work with formalin fixed paraffin embedded sections. If there is anything else I can help you with, please let me know.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"