CytoPainter Lysosomal Staining Kit - Green Fluorescence (Ex405nm) (ab112138)

Overview

  • Product nameCytoPainter Lysosomal Staining Kit - Green Fluorescence (Ex405nm)
    See all Lysosome kits
  • Tests
    1 x 500 test
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeCell-based
  • Product overview

    Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.

    ab112138 is designed to label lysosomes in live cells with green fluorescence of large Stokes Shift at Ex/Em = 405/520 nm. ab112138 uses a proprietary lysotropic dye that selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator, a hydrophobic compound, easily permeates intact live cells and gets trapped inside the lysosomes. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components. It is suitable for both suspension and adherent cells.

  • Notes

    ab112138 should be stored desiccated.

  • Tested applicationsSuitable for: FM, ICC/IFmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab112138 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    FM Use at an assay dependent concentration.
    ICC/IF Use at an assay dependent concentration.

    CytoPainter Lysosomal Staining Kit - Green Fluorescence (Ex405nm) images

    • Image of U2OS cells stained with ab112138, 405 nm Excitation in a black 96-well plate

    Protocols

    References for CytoPainter Lysosomal Staining Kit - Green Fluorescence (Ex405nm) (ab112138)

    ab112138 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting Abcam. The answers to your questions are below: 1. This is basically for all our standard alone fluorescent dyes. For this typical kit, simply store at -20C will be fine (the lyso sensor is DMSO stock solution). 2. We d...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"