Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
ab112138 is designed to label lysosomes in live cells with green fluorescence of large Stokes Shift at Ex/Em = 405/520 nm. ab112138 uses a proprietary lysotropic dye that selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator, a hydrophobic compound, easily permeates intact live cells and gets trapped inside the lysosomes. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components. It is suitable for both suspension and adherent cells.
ab112138 should be stored desiccated.
|Live Cell Staining Buffer||1 x 50ml|
|LysoGreen StrK Indicator||1 x 100µl|
Our Abpromise guarantee covers the use of ab112138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FM||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
ab112138 has not yet been referenced specifically in any publications.