Anti-Cytoplasmic dynein 1 light intermediate chain 1 antibody - N-terminal (ab185994)

Overview

  • Product name
    Anti-Cytoplasmic dynein 1 light intermediate chain 1 antibody - N-terminal
    See all Cytoplasmic dynein 1 light intermediate chain 1 primary antibodies
  • Description
    Rabbit polyclonal to Cytoplasmic dynein 1 light intermediate chain 1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Cow, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human Cytoplasmic dynein 1 light intermediate chain 1 aa 1-50 (N terminal). The exact sequence is proprietary. NP_057225.2
    Sequence:

    MAAVGRVGSF GSSPPGLSST YTGGPLGNEI ASGNGGAAAG DDEDGQNLWS


    Database link: Q9Y6G9

  • Positive control
    • HeLa, 293T, and Jurkat whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab185994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 57 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes. Probably involved in the microtubule-dependent transport of pericentrin. Is required for progress throuh the spindle assembly checkpoint. The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores.
  • Sequence similarities
    Belongs to the dynein light intermediate chain family.
  • Post-translational
    modifications
    Phosphorylated during mitosis but not in interphase.
  • Cellular localization
    Cytoplasm. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle pole.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytoplasmic dynein 1 light intermediate chain 1 antibody
    • cytosolic antibody
    • DC1L1_HUMAN antibody
    • DLC-A antibody
    • DNCLI1 antibody
    • dync1li1 antibody
    • Dynein cytoplasmic 1 light intermediate chain 1 antibody
    • Dynein cytoplasmic light intermediate polypeptide 1 antibody
    • Dynein light chain A antibody
    • Dynein light intermediate chain 1 antibody
    • Dynein light intermediate chain 1 cytosolic antibody
    • FLJ10219 antibody
    • LIC1 antibody
    see all

Images

  • All lanes : Anti-Cytoplasmic dynein 1 light intermediate chain 1 antibody - N-terminal (ab185994) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 57 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation. ab185994 at 0.1 µg/ml staining Cytoplasmic dynein 1 light intermediate chain 1 in 293T whole cell lysate immunoprecipitated using ab185994 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: controle IgG.

References

ab185994 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab185994.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up