Cytosol/Particulate Rapid Separation Kit (ab65398)

Overview

  • Product nameCytosol/Particulate Rapid Separation Kit
  • Tests
    50 x 1 assay
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay typeDirect
  • Assay time
    1h 00m
  • Product overview

    Abcam's Cytosol/Particulate Rapid Separation Kit physically separate cytosol from particulate compartments rapidly through an oil layer and thus the two fractions would not contact or diffuse to each other. Using the method, contaminations can be avoided even for small molecules. Subcellular localization and analyses of factors interested can be performed accurately.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    The location and translocation of proteins, signaling molecules, and other small molecules inside cells regulate cell growth, differentiation and many other cellular functions. Separating cytosol and particulate fractions is an important step in studying subcellular localization of cellular components. However, traditional methods usually take hours to separate cytosol from particulates, so that some cellular components especially small molecules and metabolites are getting diffused or redistributed during the separation procedure.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • RelevanceCytosol/Particulate separation is the method for separating cytosol and particulate fractions. The location and translocation of proteins, signaling molecules, and other small molecules inside cells regulate cell growth, differentiation and many other cellular functions. Separating cytosol and particulate fractions is an important step in studying subcellular localization of cellular components.

Applications

Our Abpromise guarantee covers the use of ab65398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Protocols

References for Cytosol/Particulate Rapid Separation Kit (ab65398)

ab65398 has not yet been referenced specifically in any publications.

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Particulate fractions are the unsoluble fractions of lysaed cells e.g. plasma membrane. You are correct!

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"