CyTRAK Orange™ 50µl (5 mM) (ab109203)

Overview

  • Product nameCyTRAK Orange™ 50µl (5 mM)
  • Tested applicationsSuitable for: FM, Flow Cyt, ICC/IFmore details
  • General notesCyTRAK Orange™ is a novel orange fluorescent dye [related to DRAQ5™ (ab108410)] that stains both nucleus and cytoplasm. It is water soluble and membrane permeant which can be used in live and fixed cells in combination with other common fluorophores, such as GFP-fusions, green-labelled antibodies (FITC or DyLight® 488) and far-red dyes.
    CyTRAK Orange™ preferentially stains the nucleus, but also defines the cytoplasmic area in both live and fixed preparations. CyTRAK Orange™ does the work of two fluorophores!
    Key features of CyTRAK Orange™ include:
    • Rapid staining of dsDNA/nuclei of LIVE or fixed cells
    • Differential intensity of DNA binding and Cytoplasmic labelling - One fluorophore doing the work of two
    • Low photobleaching effect
    • Ideal for use with GFP, FITC & DyLight® 488 and 649 labels
    • No lyse / no wash protocols
    • It is membrane-permeant

    Excitation

    • 457, 488 & 549 (Exmax 515nm)
    • similar profile to Propidium Iodide
    • co-excited with FITC/Cy2/eGFP and PE-Cy7
    • NOT excited by UV or red lasers (e.g. 633 & 647 nm)

    Emission (instrument dependent):

    • Emmax 615 nm
    • No significant spectral overlap with co-excited green fluors (eGFP, FITC, Cy2)
    • Visualize GFP-tagged temporospatial changes in live cells in a single acquisition from a 488 nm excitation

Properties

    Applications

    Our Abpromise guarantee covers the use of ab109203 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    FM Use at an assay dependent concentration.
    Flow Cyt 1/1000 - 1/300. For Nucleated Cell Gate = 5µM; For Discrimination of Arrested/ Senescent Cells = 15µM
    ICC/IF 1/1000. For microscopy counterstain = 5µM
    It is highly recommended that the concentration and labelling conditions are carefully determined by each investigator for optimal performance in the assay of interest. For more specific information about the applications, please refer to the Protocol Booklet.

    CyTRAK Orange™ 50µl (5 mM) images

    • Live cells expressing eGFP/CyclinB1 were labelled with CyTRAK Orange™ (end concentration 20µM) for 20 minutes at 37 C, no wash. Image was captured using confocal microscopy at 488nm simultaneously: i) CyTRAK Orange™ channel - 598/40nm; ii) eGFP/CyclinB1 channel - 522/35nm

    Protocols

    References for CyTRAK Orange™ 50µl (5 mM) (ab109203)

    ab109203 has not yet been referenced specifically in any publications.

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    Thank you for your enquiry. This CyTRAK Orange (ab109203) is a novel orange fluorescent dye [related to DRAQ5™ (ab108410)] that stains both nucleus and cytoplasm. Please rest assured that it too can be used in live cell imaging. However, we have no...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"