This clone has been derived from hybridization of Sp 2/0 myeloma cells with spleen cells of Balb/c mice immunized with homogenized fibrin clot.
Our Abpromise guarantee covers the use of ab10050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution.
To be analyzed in sandwich immunoassay, plasma must be diluted with 10 mM Tris/HCl buffer, pH 7.5, containing 1 M NaCl and 0.1 % Tween 20. To avoid non-specific binding, the final NaCl concentration in plasma samples must be 0.5 M or more.
|Sandwich ELISA||Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [DD4] to D-Dimer (HRP) (ab10054) and Mouse monoclonal [DD5] to D-Dimer (HRP) (ab10055) and Mouse monoclonal [DD6] to D-Dimer (HRP) (ab24474). Can be used as Capture antibody. Recommended for D-Dimer detection in Human Plasma.|
Lane 1 - D-Dimer
Lane 2 - D-monomer
Lane 3 - fibrinogen
Lane 4 - D-Dimer treated with mercaptoethanol