Anti-D-glucosamine antibody (ab62666)
Key features and details
- Rabbit polyclonal to D-glucosamine
- Suitable for: ICC
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-D-glucosamine antibody -
Description
Rabbit polyclonal to D-glucosamine -
Host species
Rabbit -
Specificity
Fixed tissue cross-reactivity tested with known targets at recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against any free D or L amino acid. The IgG is highly specifc for glucosamine (L/D differences not determined yet). As most other pentoses are unfixable (lacking primary amino groups), this antibody cannot detect them in fixed tissues. -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
D-glucosamine cross-linked to purifed fraction V bovine serum albumin with glutaraldehyde.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Thimerosal (merthiolate)
Constituents: PBS, 1% Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Notes |
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Endogenous content mapping by LM and EM immunocytochemistry. True dilution at user dilution: 1/2000. Optimal fixation: 0.1-2.5% glutaraldehyde,1% formaldehyde using HPI (High Performance Immunocytochemistry). Minimum glutaraldehyde: 0.05% using EHPI (Enhanced HPI) with 4% formaldehyde.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
D-glucosamine, or glucosamine-6-phosphate is the biochemical precursor of all nitrogen-containing sugars. It is synthesized from fructose-6-phosphate and glutamine as the first step of the hexosamine biosynthesis pathway. The end-product of this pathway is UDP-N-acetylglucosamine (UDP-GlcNAc), which is then used for making glycosaminoglycans, proteoglycans, and glycolipids. -
Alternative names
- Glucosamine 6 phosphate antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab62666 has been referenced in 1 publication.
- Dai N et al. Evaluation of UDP-GlcN derivatives for selective labeling of 5-(hydroxymethyl)cytosine. Chembiochem 14:2144-52 (2013). PubMed: 24106095