Overview

  • Product name
    D. melanogaster ISWI (acetyl K753) peptide

Description

  • Nature
    Synthetic

Specifications

Our Abpromise guarantee covers the use of ab16064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-ISWI (acetyl K753) antibody (ab10748)

  • Form
    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • CHRAC 140 kDa subunit
    • dISWI
    • Imitation SWI
    • NURF
    • NURF 140
    • Protein imitation swi
    see all
  • Relevance
    ISWI is a component of the nucleosome remodeling factor complex (NURF), a protein complex that facilitates the perturbation of chromatin structure in vitro in an ATP-dependent manner. The hydrolysis of ATP during the remodeling of chromatin is likely to be mediated by ISWI, releasing inorganic phosphate. It is also a component of the ATP-utilizing chromatin assembly and remodeling factor (ACF) and of the chromatin accessibility complex (CHRAC). This subunit may serve as the energy-transducing component of chromatin-remodeling machines.
  • Cellular localization
    Nuclear

References

This product has been referenced in:
  • Yang L  et al. PKM2 regulates the Warburg effect and promotes HMGB1 release in sepsis. Nat Commun 5:4436 (2014). Functional Studies ; Mouse . Read more (PubMed: 25019241) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab16064.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up