D-Sorbitol Assay Kit (Colorimetric) (ab118968)

Overview

  • Product name
    D-Sorbitol Assay Kit (Colorimetric)
  • Sample type
    Cell Lysate
  • Assay type
    Quantitative
  • Sensitivity
    > 0.1 nmol/well
  • Range
    0.1 nmol/well - 10 nmol/well
  • Assay time
    0h 40m
  • Product overview

    Abcam's D-Sorbitol Assay Kit (Colorimetric) is designed to measure sorbitol in a variety of samples such as foods, fruits, fruit juices, pharmaceuticals, cosmetics, paper and some other biological samples. In the assay, sorbitol is oxidized to fructose with the proportional development of intense color with an absorbance maximum at 560 nm. The assay is useful over the range of 0.1-10 nmol of Sorbitol per sample.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Sorbitol is one of the 6 carbon sugar alcohols. It is commonly used as an artificial sweetener, as a laxative and in cosmetics as a humectant and thickening agent. Sorbitol is produced naturally in a variety of fruits. It can be produced in humans in small amounts by the reduction of glucose by aldose reductase. Due to its poor ability to diffuse across the cell membrane, sorbitol can be trapped in cells and is believed to be one of the causes of damage (due to osmotic effects) in diabetes. Interestingly, sorbitol can be used as a screen for the O154:H7 strain of E. coli, since this strain is one of the few strains which cannot metabolize sorbitol.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab118968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • D-Sorbitol measured in various samples showing nmol/mg (fruits) and nmol/mL (juice).  300 mg (wet weight) of prune and pear was homogenised in 1 mL of water. Juice was diluted in water. Samples were diluted 2-1000 fold.

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Example of standard curve obtained using ab118968.

Protocols

References

ab118968 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry.

I can confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products. However, in ...

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Thank you for your enquiry.

I can confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products.

Ho...

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Thank you for contacting us. Unfortunatley we do not  know the cause as to why these sample types do not perform in our kit. I have found an assay published at the following link: http://www.sciencedirect.com/science/article/pii/S0009898104005455 ...

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Thank you for contacting us. As it goes with any kit there can be compatible and incompatible samples. We have tested this kit with plasma, serum etc. and knows that it doesn’t work; we do not want our customer to spend time optimizing this kit for s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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