The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/100 for 10 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
WB: Use at a concentration of 1 µg/ml for 2hours. Predicted molecular weight: 23 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRegulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:8356058, PubMed:7512369). Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133).
Tissue specificityDetected in bone marrow, thymus and spleen.
Sequence similaritiesBelongs to the Rho GDI family.
Predicted band size : 23 kDa Observed band size : 23 kDa
References for Anti-D4 GDI antibody (ab15198)
This product has been referenced in:
Weitzel LR et al. Discovery and verification of protein differences between Er positive/Her2/neu negative breast tumor tissue and matched adjacent normal breast tissue. Breast Cancer Res Treat124:297-305 (2010).
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