The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/250 - 1/500.
1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 22 kDa).
1/50 - 1/100.
Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:8356058, PubMed:7512369). Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133).
Rho GDP dissociation inhibitor (GDI) beta antibody
Rho GDP dissociation inhibitor 2 antibody
Rho GDP dissociation inhibitor beta antibody
Rho GDP-dissociation inhibitor 2 antibody
Rho-GDI beta antibody
Immunocytochemistry/ Immunofluorescence - Anti-D4 GDI antibody [EPR14212(B)] (ab181252)Image is courtesy of an Abreview submitted by Dr Armen Petrosyan.
Immunocytochemical immunoflurescence analysis of formaldehyde-fixed HEPG-2 cells, labelling D4 GDI with ab181252 at a dilution of 1/50 incubated for 3 hours at 22°C in 1% donkey serum in 0.1% PBST. Permeablization was with 0.2% Triton X-100. Blocking was with 1% donkey serum in 0.1% PBST for 1 hour at 22°C. Secondary used was a Donkey anti-rabbit polyclonal Alexa Fluor® 594 conjugate used at 1/200.