D4 GDI overexpression 293T lysate (whole cell) (ab94194)

Overview

  • Product name
    D4 GDI overexpression 293T lysate (whole cell)
  • Description
    D4 GDI overexpression lysate
  • General notes
    ab94194 is a 293T cell transfected lysate in which Human D4 GDI has been transiently over-expressed using a pCMV-D4 GDI plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Function: Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:8356058, PubMed:7512369). Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133). Similarity: Belongs to the Rho GDI family. Tissue specificity: Detected in bone marrow, thymus and spleen.

Applications

Our Abpromise guarantee covers the use of ab94194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94194 at 15µg/lane on an SDS-PAGE gel
  • All lanes : Anti-D4 GDI antibody (ab89419) at 1/500 dilution

    Lane 1 : D4 GDI overexpression 293T lysate (whole cell) (ab94194)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94194 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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