D4 GDI overexpression 293T lysate (whole cell) (ab94194)

Overview

  • Product nameD4 GDI overexpression 293T lysate (whole cell)
  • Description
    D4 GDI overexpression lysate
  • General notesab94194 is a 293T cell transfected lysate in which Human D4 GDI has been transiently over-expressed using a pCMV-D4 GDI plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:8356058, PubMed:7512369). Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133). Similarity: Belongs to the Rho GDI family. Tissue specificity: Detected in bone marrow, thymus and spleen.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

D4 GDI overexpression 293T lysate (whole cell) images

  • ab94194 at 15µg/lane on an SDS-PAGE gel
  • All lanes : Anti-D4 GDI antibody (ab89419) at 1/500 dilution

    Lane 1 : D4 GDI overexpression 293T lysate (whole cell) (ab94194)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for D4 GDI overexpression 293T lysate (whole cell) (ab94194)

ab94194 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"