Anti-HDAC1 antibody - ChIP Grade (ab1767)

Overview

• Product nameAnti-HDAC1 antibody - ChIP GradeSee all HDAC1 primary antibodies ...
• Description
  Rabbit polyclonal to HDAC1 - ChIP Grade
• Tested applicationsICC/IF, ChIP, IP, WB more details
• Species reactivity
  Reacts with: Fruit fly (Drosophila melanogaster)
  
• Immunogen

  Synthetic peptide: C-SGSGTGSGAGAKGAKENNI, corresponding to amino acids 503-521 of Fruit fly (Drosophila melanogaster) HDAC1

  Run BLAST with Run BLAST with

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferserum with 0.02% sodium azide
• PurityWhole antiserum
• Primary antibody notes The SIN3/RPD3 deacetylase complex is essential for G(2) phase cell cycle progression and regulation of SMRTER corepressor levels.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • HDACs

  • Cardiovascular
  • Heart
  • Hypertrophy
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation
  • HDACs
  • Class I

  • Cell Biology
  • Apoptosis
  • Nucleus
  • Other

Applications

Target

• FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
• Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
• Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
• Post-translational
  modificationsSumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
  Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
  Ubiquitinated by CHFR, leading to its degradation by the proteasome.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q13547 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 38565 Fruit fly (Drosophila melanogaster)

• Alternative names
  DKFZp686H12203 antibodyGON 10 antibodyHD 1 antibody

  HD1 antibodyHDAC 1 antibodyHDAC1 antibodyhdac1: histone deacetylase 1 antibodyHDAC1_HUMAN antibodyHistone deacetylase 1 (HD1) antibodyHistone deacetylase 1 antibodyReduced potassium dependency yeast homolog like 1 antibodyreduced potassium dependency yeast homolog-like 1 antibodyRPD3 (reduced potassium dependency yeast homolog) like 1 antibodyRPD3 (reduced potassium dependency) antibodyRPD3 antibodyRPD3L1 antibody

  see all

Anti-HDAC1 antibody - ChIP Grade images

• 

  IHC - Wholemount - Anti-HDAC1 antibody - ChIP Grade (ab1767)Image from Fitzsimons HL et al., PLoS One. 2011;6(12):e29171. Epub 2011 Dec 15. Fig 1.; doi:10.1371/journal.pone.0029171 December 15, 2011, PLoS ONE 6(12): e29171.
  Immunohistochemical analysis of whole mount Drosophila melanogaster brain, staining HDAC1 with ab1767, at 1/1000 dilution.