Overview

  • Product name
  • Description
    Rabbit polyclonal to DAAM1
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the N terminal region of human DAAM1.

  • Positive control
    • WB: 293 cell lysate IHC-P: human brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab71327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/10 - 1/50.
WB 1/50 - 1/100. Detects a band of approximately 130 kDa (predicted molecular weight: 123 kDa).

Target

  • Relevance
    DAAM1 (Dishevelled associated activator of morphogenesis 1) is a diaphanous-related formin which binds to dishevelled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex assembly. It is an essential component of noncanonical Wnt signaling. DAAM1 also communicates with Rho GTPases, CIP4 and Src in the regulation of the signalling pathways that co-ordinate the dynamics of the actin filament system. DAAM1 may be a coordinator of endocytosis and cytoskeletal remodeling.
  • Cellular localization
    Cytoplasmic. Note: Perinuclear.
  • Database links
  • Alternative names
    • DAAM 1 antibody
    • Dishevelled associated activator of morphogenesis 1 antibody
    • FLJ41657 antibody
    • KIAA0666 antibody

Anti-DAAM1 antibody images

  • All lanes : Anti-DAAM1 antibody (ab71327) at 1/50 dilution

    Lane 1 : 293 cell lysate, nontransfected
    Lane 2 : 293 cell lysate, transfected with the DAAM1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 123 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
  • ab71327, at a 1/10 dilution, staining DAAM1 in formalin fixed and paraffin embedded human brain tissue by Immunohistochemistry. A peroxidase conjugated secondary antibody was then used, followed by DAB staining.

References for Anti-DAAM1 antibody (ab71327)

This product has been referenced in:
  • Rydell-Törmänen K  et al. Aberrant nonfibrotic parenchyma in idiopathic pulmonary fibrosis is correlated with decreased ß-catenin inhibition and increased Wnt5a/b interaction. Physiol Rep 4:N/A (2016). IHC-P ; Human . Read more (PubMed: 26997628) »

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (htert-RPE cells)
Permeabilization
Yes - 50 mM NH4Cl and 0.1M Glycine in PBS
Specification
htert-RPE cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Oliva Palander

Verified customer

Submitted May 31 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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