The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
1/50 - 1/100. Detects a band of approximately 130 kDa (predicted molecular weight: 123 kDa).
DAAM1 (Dishevelled associated activator of morphogenesis 1) is a diaphanous-related formin which binds to dishevelled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex assembly. It is an essential component of noncanonical Wnt signaling. DAAM1 also communicates with Rho GTPases, CIP4 and Src in the regulation of the signalling pathways that co-ordinate the dynamics of the actin filament system. DAAM1 may be a coordinator of endocytosis and cytoskeletal remodeling.
ab71327, at a 1/10 dilution, staining DAAM1 in formalin fixed and paraffin embedded human brain tissue by Immunohistochemistry. A peroxidase conjugated secondary antibody was then used, followed by DAB staining.
Rydell-Törmänen K et al. Aberrant nonfibrotic parenchyma in idiopathic pulmonary fibrosis is correlated with decreased ß-catenin inhibition and increased Wnt5a/b interaction. Physiol Rep4:N/A (2016).
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